摘要
目的:建立黄麦合剂中金丝桃苷的HPLC测定方法。方法:色谱柱:Waters XBridge^(TM)C_(18)柱(250 mm×4.6 mm,5μm),流动相:乙腈-0.1%磷酸溶液(15:85),检测波长:360 nm,流速:1.0 ml·min^(-1)。结果:金丝桃苷浓度在8.42～134.72μg·ml^(-1)的范围内,峰面积与浓度呈良好的线性关系(r=0.999 9)。平均回收率为98.09%,RSD为2.79%(n=7)。结论:本法简便、准确、重复性好,可用于黄麦合剂中金丝桃苷的含量测定。
Objective：To establish an HPLC method for the determination of hyperoside in Huangmai mixtures.Method：A Waters XBridgeTM C_（18）（250 mm×4.6 mm,5μm）column was used.The mobile phase consisted of acetonitrile-1%phosphoric acid（15 ：85）.The detection wavelength was 360 nm.The flow rate was 1.0 ml · min^（-1）.Result：Hyperoside showed a good linear relationship within the range of 8.42-134.72μg· ml^（-1）（r =0.999 9）.The average recovery was 98.09%with RSD of 2.79%（n= 7）.Conclusion： The method is feasible with good specificity and reproducibility,and can be used in the determination of hyperoside in Huangmai mixtures.
出处
《中国药师》
CAS
2012年第12期1739-1740,共2页
China Pharmacist
基金
上海浦东新区科技局基金项目(编号:PKM2010-13)
