姜黄素抑制IFN-γ对HaCaT细胞促增殖作用的实验研究

Inhibition Effect of Curcumin on HaCaT Cells Proliferation Induced by IFN-γ

[目的]研究姜黄素抑制IFN-γ促人角质形成细胞株HaCaT细胞增殖的机制,为临床上姜黄素治疗银屑病奠定基础。[方法]MTT(噻唑蓝)法检测不同浓度姜黄素(0、1、5、10、20、50、100 mol.L-1)和不同浓度IFN-γ(0、1、5、10、25、50、100m ol.L-1)对HaCaT细胞增殖的影响,以及姜黄素(20mol.L-1)、Notch-1特异性抑制DAPT(5 mol.L-1)对IFN-γ(50ng.mL-1)促HaCaT细胞增殖作用的抑制情况;Western blot法检测观察20 mol.L-1的姜黄素、50ng.mL-1IFN-γ作用下HaCaT细胞Hes1和Notch-1的蛋白表达情况。[结果]姜黄素浓度依赖性抑制HaCaT细胞增殖,而IFN-γ浓度依赖性促进HaCaT细胞增殖;20 mol.L-1姜黄素及5 mol.L-1DAPT明显抑制50ng.mL-1IFN-γ对HaCaT细胞的促增殖作用,20 mol.L-1姜黄素有效抑制50ng.mL-1IFN-γ对HaCaT细胞Hes1和Notch-1蛋白的促表达作用。[结论]姜黄素有效的抑制HaCaT细胞增殖,同时可明显抑制IFN-γ对HaCaT细胞促增殖作用,Notch-1信号通路在其中可能发挥重要作用。

[Objective] To study the mechanism of Curcumin in inhibiting human keratinocyte cell line HaCaT cell proliferation induced by IFN-γ,and lay the experimental foundation for clinical use of curcumin in the treatment of psoriasis.[Methods] MTT assay was used to dectect the proliferation ability of HaCaT cells treated with different concentrations of curcumin（0,1,5,10,20,50,100mol·L-1） and different concentrations of IFN-γ（0,1,5,10,25,50,100 mol·L-1）,and the inbition effect of curcumin（20 mol·L-1） DAPT（Notch-1 specific inhibitor,5 mol·L-1） on the HaCaT cell proliferation induced by IFN-γ（50ng/ml）;western blot was used to observe the Hes1 and Notch-1protein expression of HaCaT cells influenced by 20 mol·L-1 curcumin and 50ng/ml of IFN-γ.[Results] Curcumin inhibited the proliferation of HaCaT cells in a dose-dependent manner,while IFN-γ promoted the proliferation of HaCaT cells in a dose-dependent manner;20 mol·L-1 curcumin and 5 mol·L-1 DAPT significantly inhibited the proliferation of HaCaT cells induced by 50ng/ ml.20 mol·L-1curcumin inhibited the expression of Hes1 and Notch-1 protein in HaCaT cells induced by 50ng/ml IFN-γ.[Conclusion] Curcumin can effectively inhibit the proliferation of HaCaT cells,and inhibit the proliferation of HaCaT cells induced by IFN-γ.Notch-1 signaling pathway may play an important role in it.