应用流式细胞术检测巨细胞病毒PP65抗原的研究

Application of Flow Cytometry to Detect Cytomegalovirus PP65 Antigen

目的观察人外周血有核细胞人类巨细胞病毒(human cytomegalivirus,HCMV)PP65Ag阳性表达与HCMV感染患者的相互关系。方法应用流式细胞术对广州某三甲医院137例患者(其中器官移植组14例,白血病组12例,ICU组24例,血液透析组13例,其它疾病组74例)的外周血有核细胞HCMV PP65Ag阳性表达百分率进行分析,并以本院门诊健康体检者20例为正常对照,观察26例HCMV现症感染患者HCMV PP65Ag的变化情况。结果器官移植组、白血病组、ICU组、血液透析组、其他疾病组HCMV-PP65抗原检测阳性率与正常对照组比较,均有显著性差异(P<0.05/6)。动态观察组监测结果显示,持续HCMV PP65Ag检测阳性患者,粒细胞群表达阳性时,临床有明显发热症状。结论本文研究结果表明运用流式细胞术检测人外周血有核细胞表面HCMV-PP65抗原和对HCMV感染者的细胞群进行分析,有利于临床了解疾病情况和制订治疗方案。

Objective To observe the inter-relationship between the human cytomegalovirus（HCMV） PP65Ag positive expression in human peripheral blood nucleated cells and the patients infected with HCMV.Methods The method of flow cytometry was applied to analyze the percentage of HCMV PP65Ag positive expression in peripheral blood cell nuclear in the 137 patients（14 cases of organ transplant group,12 cases of leukemia group,24 cases of ICU group,13 cases of hemodialysis group,74 cases of other disease groups） in a teaching hospital.The change of HCMV PP65Ag in 26 cases of HCMV infected patients was observed based on the normal control group of 20 healthy cases.Results Comparing with the normal control group,the positive rate of HCMV-PP65 antigen in above 5 groups showed significant difference（P 0.05 / 6）.Among the group of continuously HCMV PP65Ag testing positive patients,there was obvious clinical symptom of fever,when the granulocyte group to express positive.Conclusion The application of flow cytometry to detect the antigen HCMV-PP65 on the surface of human peripheral blood cell,and analysis of the HCMV infected cells will help to learn more about the disease and develop clinical treatment programs.