摘要
目的 探讨CD4＋CD25＋调节性T细胞与细胞因子在哮喘儿童外周血中的比例改变，并探讨其临床意义。方法分离哮喘患儿和正常人外周血单个核细胞（PBMC），用流式细胞仪分析CD4＋CD25＋调节性T细胞在外周血单个核细胞中的比例；酶联免疫吸附法（ELASA）检测外周血TGF—p和IL-10水平。结果急性发作期组患儿外周血CD＋CD25＋Tr及细胞因子水平明显低于缓解期组和正常对照组（P〈0．05），而缓解期患儿与对照组无显著性差异（P〉0．05）。结论CD4＋CD25＋调节性T细胞和细胞因子可能参与了哮喘的发生与发展。
Objective To investigate the change of CD4 ＋ CD25 ＋ T regulation cells and cytokine in peripheral blood in children with asth- ma. Methods The monouclear cells were isolated from peripheral blood of asthma children and control subjects, and the percentagn of CD4 ＋ CD25＋ regulatory T cells was detemined by flow eytonetry. The concentrations of TGF-β and IL- 10 were measured with enzyme linked immunosorbent assay（ELlSA）method. Results The CD4＋ CD25 ＋ Tr and cytokine levels in acute atack group were lower than in remission group or in the control group（ P 〈 0. 05）, but no difference between in the remission and the control （P 〉 0.05）. Conclusion The CD ＋ CD25 ＋ regulatory T cells involved in the pathogenesis of asthma in human.
出处
《医学检验与临床》
2012年第6期48-49,共2页
Medical Laboratory Science and Clinics
