乌鲁木齐市妇女感染人乳头瘤病毒的基因型研究

Characterization of Human Papillomavirus Genotype of Women in Urumqi

目的研究新疆鸟鲁木齐市妇女感染人乳头瘤病毒（HPV）的墓因型，为HPV感染防治提供依据。方法于2008年8月～2012年3月期间，收集新疆乌鲁木齐市妇女疑似HPV感染病例标本594例，应用PCR-反向点杂交（PCR—RD—BH）技术进行HPV基因扩增并分型，分析不同HPV基因型检出率，计算不同年龄组及不同妇科疚病组人群中的HPV感染率，组间率的比较采用X2检验。结果从594例病例标本中检出HPV阳性标本30l例，总阳性率为50．67％，检出21种HPV基因型，包括4种低危型HPV（HPV6，11，43和42）和17种高危型HPV（HPVl6，58，52，66，18，3l，33，56，68，59，35，53，45，51，39，73和83），其中HPVl6，i1，58，43和HPV6亚型的感染率较高；检出单重感染205例，多重感染96例。年龄分组显示19～25岁和3l～35岁两个年龄段人群HPV检出率较高，而41～45岁年龄段人群HPV阳性率最低；疾病分组统计显示，尖锐湿疣和宫颈癌患者中HPV阳性率均显著高于宫颈炎组和宫颈糜烂疾病组（P〈O．05）。结论该地区宫颈病变患者申HPV威染率较高、HPV基因型多．

Objective To genotype the human papillomaviruse（HPV） of women in Urumqi of Xinjiang and provide HPV gen- otype information for the treatment and prevention of HPV infection. Methods A total of 594 clinical specimens were col- lected between 2008 and 2012,and PCR-Reverse dot blot hybridisation （PCR-RDBH） was performed to genotype HPV in clinical specimens of 594 women with symptoms of gynecologic disorders. HPV infection rates were calculated and statistical analysis of X2 test was performed to compare the HPV infection rates between different groups with different ages and dif- ferent diseases. Results A total of 301 specimens were identified to be HPV positive and yielded a positive rate of 50.67% （301/594） . 21 kinds of HPV types including 4 low-risk HPV types （HPV6,11,43 and 42）and 17 high-risk HPV types （HPV16,58,52,66,18,31,56,68,59,35,53,45,51,39,73 and 83） were identified,and HPV16,11,58,43 and 6 were the prevalent HPV types. Additionally,206 specimens were single infection and 96 individuals were infected with multiple HPV infection. Statistic analysis shown that patients aged from 19 to 25 and also 31 to 35 had higher HPV infection rates,addi- tionally, patients aged from 41 to 45 had lower HPV infection rate. Furthermore, patients with condyloma and cervic cancer had significantly higher HPV positive rates than patients with cervicitis or cervical erosion （P〈0.05）. Conclusion HPV in- fection was common and 21 kinds of HPV subtypes could be detected in the population with different uterine-cervical disea- ses in Urumqi of Xinjiang.