雅培I2000srCMIA法筛查梅毒螺旋体特异性抗体的测量阈值探讨

Study on Measurement Threshold of Screening Specific Antibody of Treponema Pallidum with Abbott I 2000sr CMIA Method

目的对梅毒螺旋体特异性抗体筛查结果进行回顾分析，探讨改变雅培12000sr筛查梅毒螺旋体特异性抗体的Cutoff值的可能。方法收集经雅培12000sr检测的梅毒螺旋体特异性抗体阳性标本410倒并且记录其S／CO值结果，通过TPPA法做补充试验。结果通过TPPA法补充试验得出，410例标本中真阳性标本319例，真阳性率为77．8％，其中s／co值1～2之间真阳性率为19．3％，2～3之间真阳性率为50．0％，3～5之间真阳性率为84．8％，5～7之间真阳性率93．9％。7～10之间真阳性率为97．5％，≥10真阳性标本为100．0％。结论通过TPPA补充试验得出，S／CO值≥10的标本出现假阳性率为0，S／CO值〈10的标本可能出现假阳性。故当仪器检测出的s／cO值≥lO无需做补充试验可直接发结果。S／CO值〈10时应做TPPA补充试验。此次研究有利于提高实验室筛查梅毒螺旋体特异性抗体报告质量，即可减少发出的假阳性报告。

Objective To analyze retrospectively the results of screening treponema pallidum antibody, and investigate the change of Abbott I2000sr screening of specific antibody of Treponema pallidum Cutoff values. Methods Collected Trepone- ma pallidum antibody positive specimens by Abbott I2000sr screening from 410 patients and recorded the results of S/CO value,and then used the TPPA to do supplementary test. Results Through the TPPA method experiment,410 specimens of true positive specimens from 319 cases of true positive rate was 77.8%o ,in which the S/CO value 1～2 between true positive rate was 19.3% ,2～3 between true positive rate was 50%,3～5 between true positive rate was 84.8% ,5～7 between true positive rate was 93.9 %, from 7～10 between true positive rate was 97.50%, ≥10 true positive specimens for 100 %. Conclu- sion By TPPA supplementation experiment,S/CO≥10 specimens appeared a false positive rate of 0,S/CO（10 specimens would be false positive. When the S/CO≥10 without supplemental test results could be directly issued. TPPA complementa- ry tests should be done when S/CO〈10. This study is helpful to improve the laboratory screening of specific antibody of Treponerna nallidum renorr nu,qlitv.can reduce the false nositive resort issued.