高尔基体蛋白73在原发性肝癌中的表达及其临床意义

Expression of golgi protein 73 in primary hepatocellular carcinoma and its clinical significance

【目的】研究高尔基蛋白73(GP73)在原发性肝癌组织中的表达及其临床意义。【方法】采用免疫组织化学法检测47例原发性肝癌、35例癌旁及19例正常肝脏组织中的GP73,同法检测肝癌组织中的AFP。【结果】GP73在原发性肝癌、癌旁及正常肝脏组织中的阳性率分别为80.9%、34.3%、5.3%,肝癌组织中GP73阳性率与其他组织比较,差异有统计学意义(P<0.05);GP73表达与肝癌是否合并有肝硬化及淋巴结转移有关(P<0.05);肝癌组织中GP73阳性率为80.9%,AFP阳性率为59.6%;GP73和AFP两者联检敏感性有显著提高,达95.7%,与单一GP73或AFP相比敏感性有显著差异(P<0.05,P<0.01),而特异性无显著差异(P>0.05)。【结论】肝癌组织中GP73表达增加,GP73可能与肝癌的发生发展有关,GP73和AFP联合检测对肝癌的早期诊断具有良好的临床应用价值。

[Objective] To explore the expression of golgi protein 73 （GP73） inprimary hepatocellular carcinoma （PHC） and its clinical significance.[Methods] The expression of GP73 and AFP in specimens of 47 PHC, 35 tumor adjacent specimens and 19 liver normal tissues was evaluated by immunohistochemistry. [Results] The positive rate was 80.9%, 34.3%, 5.3% of GP73 in PHC respectively, tumor adjacent specimens and liver normal tissues （P 0.05）. PHC tissues GP73 positive rate compared with other tissues, and the difference was statistically significant （P 0.05）.The expression of GP73 was correlated with cirrhosis of the liver and lymph node metastasis （P 0.05）. The sensitivity of combined examnination of GP73 and AFP was 95.7%and was significantly highter than that of single GP73 or AFP test（P 0.05, P 0.01）,but the specificity of combined examniation was only slightly lower（P 0.05）. [Conclusions] The expression of GP73 was increased in PHC, and it may be associated with the development of PHC. GP73 and AFP determination in the early diagnosis of liver cancer have good clinical value.