膜性肾病小鼠模型的建立与鉴定

Establish and identification of mouse model of membranous nephropathy

目的建立小鼠膜性肾病模型。方法取6周龄健康雄性BALB/c小鼠20只,分为对照组和模型组。模型组小鼠皮下注射0.2 mg阳离子牛血清白蛋白(C-BSA)和等体积的完全弗氏佐剂,对照组皮下注射生理盐水和完全弗氏佐剂。2周后,模型组小鼠尾静脉注射阳离子牛血清白蛋白,每周3次,隔日注射,对照组使用生理盐水代替。模型的鉴定则采用血液生化检查和病理检查(PASM染色、IgG免疫荧光染色、透射电镜)等方法。结果 8周后12只模型组小鼠出现高蛋白尿、低蛋白血症和高胆固醇血症;PASM染色显示出类似于早期膜性肾病,IgG免疫荧光染色显示出沿毛细血管壁呈颗粒状物沉积,透射电镜下有足突的广泛融合。结论 C-BSA尾静脉注射小鼠可成功构建小鼠膜性肾病模型。

Objective To establish mouse models of membranous nephropathy（MN）,and identify the physiological and histological characteristics of the models.Methods BALB/c mice（6 weeks old） were randomly divided into 2 groups： an experimental group and a control group.Animals in the experimental group were immunized subcutaneously with 0.2 mg cationized bovine serum albumin（C-BSA） emulsified in an equal volume of complete Freunds adjuvant（CFA）,and those in the control group were immunized with CFA and normal saline.Two weeks later,these mice in the experimental group received C-BSA via tail vein every other day,3 times per week,and the control group received normal saline alone in the same way.The establishment of models was verified by serum,urine tests and pathological examinations（PASM staining,IgG immunofluorescence and transmission electron microscopy）.Results Eight weeks later,12 animals developed hypoalbuminemia,hypercholesterolemia and severe proteinuria in the experimental group.The PASM staining showed similar as the early stage of MN in morphology.Immunofluorescence staining for the experimental group exhibited granular deposition of IgG along the capillary wall,and ultrastructurally,the basement membrance showed fusion and effacement of podocyte foot processes and the deposits under a transmission electron microscope.Conclusion We established successfully a murine model of MN induced by C-BSA.