普罗布考对动脉粥样硬化大鼠血管活性物质的影响

Effect of probucol on vasoactive substance in rats with atherosclerosis

目的探讨普罗布考对动脉粥样硬化(AS)大鼠血管活性物质的影响。方法 60只SD大鼠随机均分为对照组(A组)、高脂饲料+维生素D3(VitD3)组(B组)、高脂饲料组(C组)、高脂饲料+普罗布考+VitD3组(D组)、高脂饲料+普罗布考组(E组)。第12周时对大鼠取血检测血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、空腹血糖(FBG)、空腹胰岛素(FIns)、一氧化氮(NO)、一氧化氮合酶(NOS)、超氧阴离子(O2-)和超氧化物歧化酶(SOD);并计算胰岛素抵抗指数(HOMA-IR),免疫组化法检测基质金属蛋白酶9(MMP-9)表达。结果与A组相比,B、C组TG、TC、LDL-C、NO、NOS、O2-、FBG、FIns、HOMA-IR水平以及MMP-9表达增加(P<0.01),HDL-C水平降低(P<0.01);而普罗布考干预后NO、NOS水平升高(P<0.05),TC、HDL-C、LDL-C、O2-水平以及MMP-9表达减少(P<0.05),TG、SOD、FBG、FIns、HOMA-IR水平无统计学差异(P>0.05)。结论普罗布考通过抑制炎症反应,减少活性氧簇的生成,进而改善AS大鼠的血管内皮功能。

Objective To investigate the effect of probucol on vasoactive substance in rats with atherosclerosis. Methods Sixty SD rats were equally randomized into five groups of A（normal control）,B（high fat diet＋vitamin D3）,C（high fat diet）,D（high fat diet＋vitamin D3＋probucol） and E（high fat diet＋probucol）.On the 12th week,the blood samples were collected for detecting triglyce-ride（TG）,total cholesterol（TC）,high density lipoprotein cholesterol（HDL-C）,low density lipoprotein cholesterol（LDL-C）,fasting blood glucose（FBG）,fasting insulin（FIns）,nitric oxide（NO）,nitric oxide synthase（NOS）,O-2,superoxide dismutase（SOD） and insulin resistance index（HOMA-IR）.The expression of matrix metalloproteinase-9（MMP-9） was measured by immunohistochemistry. ResultsCompared with group A,the levels of TG,TC,LDL-C,NO,NOS, O-2,FBG,Fins,HOMA-IR and the expression of MMP-9 were increased（P〈0.01）,while the level of HDL-C was decreased in groups of B and C（P〈0.01）.However, probucol could elevate the levels of NO and NOS（P〈0.05）,reduce the levels of TC,HDL-C,LDL-C and O-2 and the expression of MMP-9（P〈0.05）,but not obviously change the levels of TG,SOD,FBG,FIns and HOMA-IR（P〈0.05）. Conclusion Probucol can improve vascular endothelium function in rats with atherosclerosis via inhibiting inflamation reaction and ROS production.