细胞色素P450 4F2 V433M位点基因多态性与缺血性脑卒中易感性的meta分析

Meta-analysis of association between cytochrome P450 4F2 V433M polymorphisms and susceptibility of ischemic stroke

目的通过对细胞色素P4504F2（CYP4F2）V433M位点基因多态性与缺血性脑卒中的相关性进行meta分析，为缺血性脑卒中的遗传病因学提供参考依据。方法检索至2012年7月为止国内外数据库收录的有关CYP4F2V433M位点基因多态性与缺血性脑卒中关系的病例对照研究．选择最佳遗传模型，利用STATA11．0软件进行meta分析。根据人选文献的异质性检验结果选择合并数据的效应模型，计算出合并的OR值和95％CI，用Egger’S和Begg’S检验评价发表偏倚。结果共纳入5篇文献，6个人群，其中4篇文献提供了两种性别的关联结果，选择的最佳遗传模型为Recessive模型（vv”sVM＋MM）。（1）合并结果显示：与野生型W相比，携带突变杂合型VM和突变纯合型MM的个体患缺血性脑卒中的风险无显著性关联（0R合并=1．11，95％CI：0．91～1．34，P=-0．304）。（21亚组分析显示：在男性群体中，CYP433M位点基因多态性与缺血性脑卒中的患病风险有显著性关联（OR=1．37，95％CI：1．16～1．60，P=-0．000），但在女性群体中，差异无统计学意义（P〉0．05）；无论是亚洲人种还是高加索人种，CYP4F2V433M位点基因多态性与缺血性脑卒中的发病风险均无显著性相关：在平均年龄〉60岁的老年人群中，CYP4F2V433M位点基因多态性能增加个体患缺血性脑卒中的风险（OR=I．20，95％CI：1．01～1．42，P=0．039），但在平均年龄〈60岁的人群中，差异无统计学意义（P〉0．05）。结论CYP4F2V433M位点基因多态性可能是男性和60岁以上老年人发生缺血性脑卒中的易感基因。

Objective To evaluate the association between cytochrome P450 4F2 （CYP4F2） V433M polymorphisms and susceptibility of ischemic stroke （IS） by means ofmeta-analysis to provide evidence for genetic etiology. Methods The case-control studies on association of CYP4F2 V433M polymorphisms and IS were retrieved in large databases fi＇om home and abroad. The software stata 11.0 was used for meta-analysis. According to the results of heterogeneity test, we selected fixed or random effect model to calculate the combined odd ratio and 95% confidence interval （95% CI）, and the publication bias was assessed by Egger＇s and Begg＇s test. Results Five eligible literatures and 6 populations were included. Recessive model （VV vs. VM ＋MM） was selected as the best genetic model. （1） Statistics of the combined data showed that as compared with that in patients with wild type VV, correlation of polymorphisms and IS in patients with heterozygosis type （VM） and homozygotic type （MM） was not significant （OR=I.11, 95%CI： 0.91-1.34, P-=-0.304）. （2） Subgroup analysis showed that the combined OR of susceptibility to IS in VM＋MM compared to W wild homozygotes was 1.37 in male, with significant difference （95%CI： 1.16-1.60, P=-0.000）, but no significance in female （P〉0.05）; no significant difference on OR of studies involving V433M was noted between Asian and Caucasoid （P〉 0.05）; in people older than 60 years, V433M polymorphism could increase the risk oflS （OR=l.2, 95%CI：1.01-1.42, P=-0.039）, but no significant difference in people 〈60 years （P〉0.05）. Conclusion CYP4F2 V433M maybe the susceptible gene oflS in male and people older than 60 years.