自发性高血压大鼠交感神经节与肾上腺髓质内NPY的表达及超微结构的变化

Changes of ultrastructure and NPY expression in sympathetic ganglion and adrenal medulla of spontaneous hypertensive rats

目的:通过对自发性高血压大鼠(SHR)颈上神经节、肾上腺髓质内神经肽酪氨酸(NPY)表达及超微结构变化的研究,探讨NPY在高血压发生和发展中的作用。方法:随机选取成年大鼠60只,分成SHR组和正常对照组,每组各30只,采用Real-Time PCR技术和免疫组织化学法,检测两组大鼠颈上神经节、肾上腺髓质内NPYmRNA和蛋白的表达;应用透射电镜技术观察上述两种组织内超微结构的改变。结果:与同周龄的正常组大鼠相比,SHR大鼠的血压明显增高(P<0.05),RT-PCR和免疫组织化学方法检测到颈上神经节、肾上腺髓质内NPYmRNA和蛋白质的表达均增高(P<0.05),NPY免疫阳性神经元的数量和阳性细胞的光密度亦增加(P<0.05)。电镜结果示SHR大鼠两种组织内异染色质、线粒体、粗面内质网等数量均明显增加(P<0.05)。结论:SHR颈上神经节、肾上腺髓质内的NPY通过复杂的机制可能参与了高血压的形成。

Objective： To investigate the effects of neuropeptide Y （NPY） in the occurrence and development of hyper- tension by observing the changes of NPY in superior cervical ganglion and adrenal medulla of spontaneous hypertensive rat （SHR）. Methods ：The adult SHR with hypertension（ n = 30）and control adult rats（ n = 30）were used. Real-Time PCR and the immunohistochemical S-P method were used to examine the expression of NPY mRNA and protein in superior cer- vical ganglion and adrenal medulla of SHR and control rats. Electron microscopy was used to examine the changes of ul- trastructure. Results：The blood pressure of SHR was obviously higher than the control group（ P 〈 0.05） ; the results of RT- PCR and the immunohistoehemistry showed that the mRNA and protein expression of NPY in superior cervical gangli- on and adrenal medulla of SHR were increased than those in control group（P 〈 0.05 ）. The results of electron microscopy showed that the mitochondrion, chondriosome, rough endoplasmic reticulum were increased significantly in superior cervi- cal ganglion and adrenal medulla of SHR （ P 〈 0.05 ）. Conclusion ： NPY in superior cervical ganglion and adrenal me- dulla of SHR may participate in the occurrence of hypertension by some complex mechanisms.