荧光原位杂交技术检测骨髓增生异常综合征患者+8和20q-

Detection of chromosomes +8 and 20q- in patients with myelodysplastic syndrome by fluorescence in situ hybridization

目的探讨荧光原位杂交(FISH)技术检测骨髓增生异常综合征(MDS)患者8号和20号染色体异常的应用。方法 40例MDS患者,采用间期FISH技术,选取+8和20q-探针的组合,检测MDS患者中的+8和20q-异常情况。结果利用FISH技术,+8和20q-检出率分别为12.5%,15.0%。采用FISH技术的组合探针可以检测出8号和20号染色体异常。结论 FISH技术能够检测出MDS染色体异常,采用组合探针的FISH更为敏感和特异。

Objective To explore the sensitivity and specificity of fluorescence in situ hybridization（FISH） technique in detection of patients with mylodysplastic syndrome（MDS）.Methods Bone marrow cells of 40 patients with MDS were analyzed by interval FISH,and the combination of probes had been used to detect the abnormalities of chromosome 8 and chromosome 20.Results Chromosome ＋8 had been found in 5 of 40 patients with MDS（12.5%）,and chromosome 20q-had been found in 6 of 40 patients with MDS（15.0%）.Conclusion The detection of abnormalities in chromosomes is helpful in diagnosis and prognosis of patients with MDS,and it is more effective and sensitive in detection of abnormalities in chromosomes by interval FISH technique.