模拟酶催化荧光分析法测定过氧亚硝酸根阴离子

Mimetic Enzyme-catalyzed Spectrofluorometric Determination of Peroxynitrite Anions

基于血红蛋白催化过氧亚硝酸根阴离子(ONOO-)氧化L-酪氨酸的反应,建立了一种测定ONOO-的新方法.在优化实验条件下,酪氨酸荧光强度随ONOO-浓度增大而猝灭,产物酪氨酸二聚体的荧光强度随ONOO-浓度增大而增强.ONOO-浓度在3.0×10-6～5.0×10-8mol/L范围内与酪氨酸的相对荧光强度呈线性关系,检出限为1.12×10-9mol/L;ONOO-浓度在3.0×10-5～3.0×10-6mol/L范围内与产物酪氨酸二聚体的相对荧光强度呈线性关系,检出限为1.31×10-8mol/L.将该方法用于样品分析,结果令人满意.

Based on a reaction of hemoglobin catalyzed the oxidation of tyrosine with peroxynitrite anions（ONOO-）,a novel method for the determination of peroxynitrite anion was developed.Under the optimized conditions,the fluorescence intensity of tyrosine was quenched and fluorescence intensity of tyrosine dimer product was enhanced with the concentration of ONOO-increasing.The linear relationship between relative fluorescence intensity of tyrosine and ONOO-concentration was in the range of 3.0×10-6~5.0×10-8 mol/L,and the detection limit was 1.12×10-9 mol/L.The linear relationship between relative fluorescence intensity of tyrosine dimer product and ONOO-concentration was in the range of 3.0×10-5~3.0×10-6 mol/L,and the detection limit was 1.31×10-8 mol/L.This method was applied to sample analysis with satisfaction.