氟尿嘧啶及顺铂对胃癌细胞microRNAs 表达谱的影响

5-Fluorouraeil and cisplatin modify expression profiles of microRNAs in human gastric cancer cells in vitro

目的通过分析氟尿嘧啶（5-Fu）联合顺铂（DDP）对胃癌细胞 microRNAs 表达谱的影响,探讨其治疗的分子机制.方法 MTT 法检测不同浓度5-Fu（终浓度分别为80、40、20、10、5、2.5μg/ml）和 DDP （终浓度分别为1.6、0.8、0.4、0.2、0.1、0.05μg/ml）作用下人胃癌细胞株9811P 增殖情况;用 IC50浓度的5-Fu和 DDP 干预9811P 细胞48h 后,利用 miRNA 芯片检测用药前后9811P 细胞 miRNAs 表达的差异;并用实时定量 RT-PCR 进行验证.结果5-Fu及 DDP 与人胃癌细胞株9811P 生长抑制率之间有浓度依赖关系,48h 的 IC50值分别为7.68、0.59μg/ml;分别用 IC50浓度的5-Fu和 DDP 干预细胞48h 后筛到29个与5-Fu干预相关 miRNAs,27个与 DDP 干预相关 miRNAs,应用实时定量 PCR 来验证了5-Fu和 DDP 组均显著上调的3个 miRNAs （miR-31、miR-96和 miR-141）,结果与芯片结果一致.通过 TargetScan 等软件对 miR-96靶基因进行预测,共筛选出8个靶基因的生物学功能与胃癌相关.结论5-Fu和顺铂对胃癌细胞 miRNAs 的表达谱有影响,可能是5-Fu 和顺铂抑制胃癌细胞生长的作用途径之一.

Objective To investigate the effect of 5-fluorouracil （5-Fu） and cisplatin （DDP） on the expression profiles of miRNAs in human gastric cancer cel s. Methods The anti-tumor effect of 5-Fu and DDP on gastric cancer 9811P cel s was an-alyzed by MTT and IC50 values of both drugs were calculated. The differential expression of miRNAs were analyzed by miRNA mi-croarray, RT-PCR and verified by RT-PCR. Results IC50 values of 5-Fu and DDP for the 9811P cel s were 7.68μg/ml and 0.59μg/ml respectivelyt. Compared to controls 29 and 27 of miRNAs in gastric cancer 9811P cel s were identified as differential y expressed after exposed to 5-Fu or cisplatin, respectively. miR-31, miR-96, miR-141 were up-regulated in cel lines both after treatment of 5-Fu or DDP, which were validated by RT-PCR. Prediction of miR-96 with TargetScan software revealed that 8 genes might be related to biological behavior of gastric cancer. Conclusion 5-Fu and DDP can modify the expression profiles of miRNAs in gastric cancer cel s, which may be partial y associate with their pharmacological effects