棘孢木霉丝裂原活化蛋白激酶基因task1的克隆及序列分析

Cloning and Sequence Analysis of Trichoderma asperellum Mitogen-Activated Protein Kinase(MAPK) Gene Task1

为深入研究丝裂原活化蛋白激酶(Mitogen-activated protein kinase,MAPK)的功能,从棘孢木霉(Tricho-derma asperellum)中克隆了丝裂原活化蛋白激酶(MAPK)基因task1,并对其序列进行分析。该基因编码355个氨基酸,全长1 757 bp,理论分子质量41.1 kD,理论等电点为6.64,与深绿木霉(T.atroviride)MAPK基因tmk1、里氏木霉(T.reesei)MAPK基因tmkA和绿色木霉(T.virens)MAPK基因tmkA在氨基酸和核苷酸水平上同源性都很高,蛋白结构预测为丝氨酸/苏氨酸蛋白激酶。

We cloned and analyzed mitogen-activated protein kinase （MAPK） gene taskl from Trichoder- ma asperellum for further studying the mitogen-activated protein kinase （MAPK） function. The whole length of the gene taskl was 1 757 bp, encoding 355 amino acids, theoretical molecular weight was 41.1 kD and theoretical isoelectric point was 6.64. The gene taskl has high homology with Tr/choderma atroviride MAPK gene tmkl, Tr/ehoderma reesei MAPK gene trnkA and Trichoderma virens MAPK gene tmkA in amino acid and nucleotide level. The protein structure prediction of gene taskl was a serine/ threonine protein kinase.