大鼠心肌缺血再灌注损伤不同时相氧化应激相关指标的变化

Changes of oxidative stress indicators at different time phases of myocardial ischemia-reperfusion injury in rats

目的检测大鼠心肌缺血再灌注损伤后不同时相氧化应激主要指标超氧化物歧化酶(SOD)活力与丙二醛(MDA)含量在血清中的变化,研究其随缺血再灌注损伤时间的变化规律及在心肌缺血再灌注损伤中发挥的作用。方法选择健康成年雄性SD大鼠12只,随机分为假手术组(S组)和缺血再灌注组(I/R组),制备大鼠心肌缺血再灌注模型,检测大鼠心肌缺血再灌注损伤后不同时相血清中SOD活力和MDA含量的变化,并与假手术对照组进行比较。结果与假手术对照组各时相相比较,血清SOD活力和MDA含量在心肌缺血再灌注后3h即出现明显变化,随着时间推移差异越来越显著,再灌注后24 h达到变化的峰值,随后两者变化的趋势与假手术对照组的差异逐渐减小,再灌注72 h后差异显著减小,但仍恢复不到假手术对照组的水平。且血清中SOD活力和MDA含量的变化呈负性相关:血清SOD活力先降低后升高,血清MDA含量先升高后降低。结论血清SOD活力和MDA含量的变化可以很好地反映出心肌细胞的损伤修复情况,一种新的量化指标有望在临床中推广,在缺血性心脏病的早期发现和治疗疗效评价中发挥重要作用。

Objective To detect the changes of superoxide dismutase（SOD） activity and malondialdehyde（MDA） content in serum with time after myocardial ischemia-reperfusion injury and their significance of these changes in rats.Methods Twelve healthy adult male SD rats were randomly divided into sham operation group（S group） and ischemia-reperfusion group（IR group）.Myocardial ischemia-reperfusion rat model was established.Serum SOD activity and MDA content were detected at different time points after ischemia-reperfusion and compared with the sham control group.Results Compared with sham-operated control group,changes in serum SOD activity and MDA content were found 3 h after myocardial ischemia-reperfusion,reached the peak 24h after reperfusion,and gradually decreased 72 h after reperfusion.Serum SOD activity and MDA content showed a negative correlation： serum SOD activity decreased first then increased,in contrast,serum MDA content increased first then decreased.Conclusion Serum SOD activity and MDA content are good indicators for myocardial cell injury and repair and may play an important role for the early detection of ischemic heart disease and evaluation of treatment efficacy.