复合全谷豆粗杂粮对脂代谢紊乱大鼠体内外抗氧化作用的影响

Effect of compound whole grain-soybean on oxidative stress

目的阐明复合全谷豆粗杂粮的体外抗氧化能力,及其对大鼠脂代谢紊乱和氧化应激水平的影响。方法制备复合全谷豆粗杂粮和大米、面粉、黑米提取物,用化学比色法测定其总抗氧化能力、羟自由基和超氧阴离子的抑制率。44只SPF级大鼠随机分为阴性对照组、高脂模型组、米面组和复合全谷豆粗杂粮组,分别给予相应饲料,连续喂养8周,实验前后测定各组大鼠体重、血清总胆固醇(TC)、总甘油三酯(TG)、血清高密度脂蛋白胆固醇(HDL-c),以及大鼠血清和肝脏的丙二醛(MDA)、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平。结果复合全谷豆粗杂粮的体外总抗氧化、清除羟自由基和清除超氧阴离子自由基的能力均显著高于大米和面粉。与高脂模型组和米面组相比,复合全谷豆粗杂粮组血清TC、TG显著下降,HDL-c显著上升,血清和肝脏MDA显著下降,T-AOC、SOD、GSH-Px显著上升;与阴性对照组相比未见显著差异。结论复合全谷豆粗杂粮具有良好抗氧化损伤作用,该作用是其改善脂代谢紊乱的重要功能机制之一。

Objective To observe the in vitro oxidation resistance of compound whole grain and the effect on improving the disorder of lipid metabolism and the oxidative stress in rats. Methods Make extracting of compound whole grain, rice, flour and black rice, method use chemical colorimetry to detect total antioxidant capacity, hydroxyl radical （.OH） and superoxide anion （02： ）. Forty-four male SD rats were divided into four groups in random ： negative control group, model control group, white rice-flour group and compound whole grain. All 4 groups were fed for 8 weeks with different experimental diets. Weight, total cholesterol （ TC ）, triglyceride （ TG）, high density lipoprotein cholesterol（HDL-C） were detected in serum. Malondialdehyde （MDA） , total antioxidantcapacity （T-AOC） , super oxygen dehydrogenizes （SOD） , glutathione peroxidase （ GSH- Px） were detected in serum and liver. Results The T-AOC, the ability of body cleaning hydroxyl radical and superoxide anion were enhanced,quite with the black rice. In all 3 treatment groups, compound whole grain group had higher HDL-C, T-AOC, SOD, GSH- Px, while TC, TG, MDA were lower. Compared with negative control groups, there is no significant difference. Conclusion Compound whole grain can have good effect on oxidative stress. This effect is the important mechanism of lipid metabolism disorders.