下呼吸道感染住院患儿人博卡病毒感染的临床特征

Clinical features of human bocavirus infections in hospitalized children with lower respiratory tract infections

目的研究<1岁急性下呼吸道感染患儿人博卡病毒(HBoV)感染的临床特征。方法采用实时荧光定量PCR法对2009年11月-2010年12月453例因急性下呼吸道感染的<1岁患儿鼻咽部抽吸物进行博卡病毒检测,阳性病例采用实时荧光定量PCR对其DNA载量进行测定,并结合其临床特征进行综合分析。结果 HBoV阳性检出35例,总阳性检测率为7.73%,平均年龄10.5月龄,季节性及性别差异无统计学意义;HBoV感染重症感染患儿(4.2×108拷贝/ml)与普通感染患儿(1.7×108拷贝/ml)HBoV的病毒载量差异无统计学意义;HBoV协同感染(6.5×106拷贝/ml)与独立感染(1.3×106拷贝/ml)患儿病毒载量差异也无统计学意义。结论博卡病毒是<1岁患儿下呼吸道感染的重要病原体,常年均可见HBoV检出病例,其病毒载量与临床表现严重程度无明显相关性,博卡病毒不是<1岁患儿急性下呼吸道感染的惟一因素。

OBJECTIVE To analyze the clinical features of human bocavirus（HBoV） infections in the children with acute lower respiratory tract infections aged less than one year.METHODS The detection of HboV was performed with real-time PCR for the nasopharyngeal aspirates from 453 children with less than 1 year of age who were with acute lower respiratory tract infections and enrolled the hospital from Nov 2009 to Dec 2010.The real-time PCR was employed to determine the DNA load for the postive cases,and the clinical features were comprehensively analyzed.RESULTS Totally 35 cases were detected HBoV-positive with the total positive rate of 7.73%,the average age was 10.5 months.The differences in the season and the gender were not statistically significant.HBoV viral load did not differ significantly between the common infection cases（1.7×108copies/ml）and the cases with severe HBoV infections（4.2×108copies/ml）,and there was also no significant difference in the viral load between the children with synergistic HBoV infections（6.5×106copies/ml）and the children with independent infections（1.3×106copies/ml）.CONCLUSION HBoV can be detected perennially and is considered as a major pathogen associated with acute respiratory tract infections in children aged less than one yearold.The viral load is not closely related to the severity of clinical manifestations,and HboV is not the only factor for the acute lower respiratory tract infections in the childre aged less than one yearold.