人婆罗双树样基因及其蛋白在儿童急性白血病中表达的意义

Significance of expression of sal-like gene 4 mRNA and protein in children＇ s acute leukemia

目的研究人婆罗双树样基因（SALL4）mRNA及其蛋白在儿童急性白血病（AL）中的表达情况。方法运用逆转录聚合酶链式反应（RT-PCR）技术对急性白血病患儿及正常对照的单核细胞进行SALL4 mRNA表达测定，运用ELISA法同步检测患儿血清中SALL4蛋白表达水平，并对其相关性进行分析。结果①AL组与对照组之间SALL4 mRNA表达率比较差异有统计学意义（P〈0．05），其中急性淋巴细胞白血病组（ALL）和急性非淋巴细胞白血病（ANLL）组均明显高于正常对照组（P〈0．05），B—ALL和T—ALL之间的SALL4 mRNA表达率比较差异有统计学意义（P〈0．05），而B—ALL与ANLL组间比较差异无统计学意义（P〉0．05）。②B．ALL组、T-ALL组、AN—LL组、正常对照组的SALL4蛋白表达量均数分别为：136±17、33±23、151±64、55±16。③各组中的SALL4 mRNA和外周血清中的SALL4蛋白表达呈正相关（r=0．931，P〈0．01）。结论儿童AL骨髓单个核细胞中存在SALIA基因的异常高表达，与健康对照比较差异有统计学意义，提示SALL4基因参与了儿童AL的发病。儿童AL中，SALL4mRNA及其蛋白在B—ALL和ANLL中高表达，且呈正相关；而在T—ALL和健康对照中不表达，提示儿童AL中B—ALL和T—ALL的发病机制可能不同；而B—ALL和ANLL可能存在同样的发病机制。

Objective To study the mRNA and protein expression of sal-like gene 4 （SALL4） in children＇s acute leukemia （AL）. Methods Through the real-time semi-quantitative reserve transcriptase-polymerase chain reaction（RT-PCR） method to test the mRNA expression level of SALL4 in the bone marrow of children＇ s AL and normal control group, explore the protein expression level of SALL4 in enzyme-linked immuno sorbent assay（ELISA）, and proceed the correlation analysis. Results （1）There was a statistically significant expression between AL and control group （ P 〈 0.05 ）, the mRNA expression levels of SALL4 in ALL and ANLL were significantly higher than those in control group （ P 〈 0. 05 ）, there was a statistically significant expression between B-ALL and T-ALL （ P 〈 0. 05 ）, but the mRNA expression level of SALIA has no difference between B-ALL and ANLL （ P 〉 0. 05 ）. （2）The amount of SALL4 protein expression in B-ALL, T-ALL, ANLL was respectively 136 ± 17, 33 ±23, 151 ±64, 55 ± 16. （3） The mRNA and protein expression of SALL4 was positively correlated（ r =0. 931, P 〈0.01 ）. Conclusions There is abnormal higher expression of SALL4 in children＇ s AL bone marrow mononuclear ceils, it is statistically significant compared with the normal control group, and suggest that SALIA is involved in the pathogenesis of children＇ s AL. In children＇ s AL, the mRNA and protein expression of SALL4 is higher in B-ALL and ANLL, and positively related, but they don＇t express in T-ALL and the normal control group. It suggests that the pathogenesis in children＇ s B-ALL and T-ALL may be different, and the pathogenesis in children＇ s B-ALL and ANLL may be same.