转移抑制基因、基质金属蛋白酶-2在食管鳞状细胞癌中表达的意义

Expression of nm23-H1 gene product and MMP-2 in esophageal squamous cell carcinoma and their clinical significance

目的探讨转移抑制基因（nm23-H1）、基质金属蛋白酶乏（MMP-2）蛋白在食管鳞状细胞癌中的表达及其临床意义。方法采用免疫组化S—P法检测nm23-H1、MMP-2蛋白在58例食管鳞状细胞癌组织和23例正常食管黏膜中的表达。结果nm23-H1蛋白在食管鳞状细胞癌中的阳性率为43．1％，在正常食管黏膜组织中的阳性率为100％，二者比较差异有统计学意义（P〈0．05）。MMP-2蛋白在食管鳞状细胞癌中的阳性率为91．4％，在正常食管黏膜组织中的阳性率为34．8％，二者比较差异有统计学意义（P〈0．05）。nm23-H1、MMP-2在食管鳞癌中的表达与患者性别、年龄及肿瘤大小无关（P〉0．05），与肿瘤的分化程度、浸润深度及有无淋巴结转移有关（P〈0．05），nm23-H1的表达与切端癌残留有关（P〈0．05）。nm23-H1与MMP-2的表达呈负相关。结论nm23-H1、MMP-2均在食管癌的发生发展过程中发挥作用，均可促进远处转移的发生；nm23-H1的表达缺失可能与切端癌残留有关；nm23-H1、MMP-2可作为判断食管癌转移及预后的指标。

Objective To investigate the expression of nm23-H1 and MMP-2 in esophageal squamous cell carcinoma（ESCC） and their clinical significance. Methods Immunochemical S-P method were used to detect the expression of nm23-H1, MMP-2 protein in 58 patients with ESCC and 23 people with normal esophageal mucosa. Results Twenty-five cases （43.1% ） were positive for nm23-H1 staining and 33 cases （56.9%） were negative. The rate of nm23-H1-positive patients was significantly less than that in normal groups whose rate was 100% （P 〈 0. 05）. Fifty-three cases （91.4%） were positive for MMP-2 staining and 5 cases （8.6%） were negative, while eight（34. 8% ） of 23 patients were positive for MMP-2 staining and 15 cases （8.6%） were negative. There was significant difference inMMP-2 positive and normal gro analysis of these of nm23-H1 and -proportion of ESCC patients ups（P 〈 0.05）. A further data shown that the expression MMP-2 was related to tumordifferentiation degree, invasion depth and lymph node metastasis （P 〈 0. 05 ）, and was not related to patients＇ gender, age and tumor size （P 〉 0.05）. The expression of nm23-H1 was related to residual cancer （ P 〈 0.05）, and was negatively correlated to expression of MMP-2. Conclusions nm23-H1, MMP-2 play a role in the development and progression of esophageal cancer, which can increase risk of distant metastasis. Residual cancer may be associated with the absent of expression of nm23-H1. The expression of nm23-H1 and MMP-2 protein can be a marker for judging esophageal cancer metastasis and prognosis.