单环刺螠废弃内脏中粗多糖的提取工艺优化

Optimization of Extraction Process for Crude Polysaccharides from Urechis unicinctus Viscera

利用热水浸提的方法进行单环刺蜢（海肠）废弃内脏中多糖的提取。采用单因素试验和L9（3^4）正交试验设计，研究不同pH值、料液比、提取时间和提取温度对单环刺蜢内脏多糖提取率的影响。并采用Fenton体系测定单环刺蜢内脏多糖对羟自由基（·OH）的清除作用。单环刺蜢内脏多糖提取的最佳工艺条件为pHl0、料液比1：40（g／mL）、时间3h、温度60℃。单环刺蜢内脏多糖在质量浓度为50μg／mL时，对羟自由基清除能力可达60％，且具有剂量效应。

Crude polysaccharides were extracted from Urech＆ unicinctus viscera using hot water extraction. One-factor- at-a-time method and an L9（34） orthogonal array design were employed to investigate the effects of pH, solid-to-solvent ratio, extraction time and temperature on extraction efficiency. In addition, the hydroxyl radical scavenging activity of polysaccharides from Urechis unicinctus viscera in a Fenton system was tested. The optimal extraction conditions were found to be 10, 1：40 （g/mL）, 3 h and 60℃ for pH, solid-to-solvent ratio, extraction time and temperature, respectively. The extract obtained under these conditions had dose-dependent scavenging activity against hydroxyl radical with a scavenging rate of 60% at a concentration of 50μg/mL.