高效液相色谱法测定养殖水体中呋喃西林

Determination of Nitrofurazone Residues in Aquaculture Water by HPLC

建立一套养殖水体中呋喃西林的高效液相色谱检测方法。结果表明液液提取不适用于水体中呋喃西林(NF2)的提取,利用固相萃取效果最佳,比较几种固相萃取小柱萃取效果,发现MCX、HLB、C_(18)对NFZ具有保留作用,MCX适合用于处理偏碱性样液,HLB处理效果受pH值影响较小,C_(18)与HLB相比效果稍差。以HLB小柱浓缩水体中呋喃西林方法,回收率在85%～95%之间,相对标准偏差小于10%,定量限达到0.25 1μg/L,可应用于各类型养殖水体中的呋喃西林残留测定。

An HPLC method for the determination of nitrofurazone （NFZ） residues in aquaculture water was established. Solid-phase extraction （SPE） rather than liquid-liquid extraction was a suitable sample preparation method for the extraction of nitrofurazone residues from aquaculture water. Although MCX, HLB and Cxs SPE cartridges had a good retention efficiency for NFZ, MCX was suitable for separating alkaline extracts, whereas HLB was minorly influenced by pH and slightly superior to C18. When NFZ was separated on HLB cartridge, the average recovery rate of the developed method was 85%-95% （n = 3）, with relative standard deviation （RSD） less than 10%. The limit of quantification （LOD） of the HPLC method was 0.25 μg/L. Thus, this method is applicable to determine residual NFZ in aquaculture water.