血管紧张素Ⅱ对人星形胶质细胞老化的影响

The effect of angiotensin Ⅱ on human astrocyte senescence

目的本研究通过体外细胞实验,拟探讨血管紧张素Ⅱ对人星形胶质细胞老化的影响。方法血管紧张素Ⅱ不同剂量(1,10,100nmol/L)刺激人星形胶质细胞3d,或者相同剂量血管紧张素Ⅱ(100nmol/L)不同时间(1、3、7d)刺激人星形胶质细胞,利用β半乳糖苷酶染色评估细胞内细胞老化。100nmol/L血管紧张素Ⅱ刺激人星形胶质细胞0、15、30、60min,利用二氢乙啡啶(DHE)染色评估活性氧产生。结果血管紧张素Ⅱ剂量依赖性引起人星形胶质细胞DHE染色表达增多,在30min达到顶峰(P<0.01)。血管紧张素Ⅱ(10,100nmol/L)也剂量依赖性引起β半乳糖苷酶染色细胞增多(P<0.01)。结论本研究揭示血管紧张素Ⅱ剂量依赖性诱导人星形胶质细胞老化,提示在脑缺血损伤和老年退行性疾病进展期,血管紧张素Ⅱ引起活性氧产生和细胞老化的内在机制。

Objective This study was conducted to determine the effect of angiotensin Ⅱ on reactive oxygen species production and cellular senescence in cultured human astrocytes. Methods Angiotensin Ⅱ （100 nmol/L） stimulated human astrocyte for 1, 3 or 7 days. Cultured human astrocyte was treated by angiotensin（1,10,100 nmol/L）for 3 days. β-galactosidase staining was used to evaluate cell senescence. Angiotensin Ⅱ （100 nmol/L） stimulated human astrocyte at 0,15,30 and 60 min. Oxidative fluorescent dihydroethidium was used to evaluate in- tracellular production of superoxide anion. Results The staining of dihydroethidium was increased in a dose-de- pendent manner by angiotensin I/ with a peak at 30 min in human astrocyte. Angiotensin Ⅱ dose-dependently induced the increase of 13-galactosidase staining cells. Conclusion Our data indicated that angiotensin Ⅱ induced superoxide production and astrocyte senescence in cultured human astrocytes：