红车轴草总异黄酮成分DNA结合剂的超滤质谱筛选

Screening DNA Binders from Isoflavone Extracts of Red Clover by Centrifugal Ultrafiltration-LC-MS^n

采用离心超滤和液相色谱-质谱联用的方法,从红车轴草异黄酮提取物中筛选DNA结合剂.结果表明,红车轴草中10种异黄酮成分与DNA具有不同的结合能力.采用液相色谱-串联质谱联用技术对其结构进行了鉴定,DNA结合能力较强的5种化合物分别是德鸢尾素-4'-O-β-D-葡萄糖苷、德鸢尾素、黄豆黄葡萄糖苷、红车轴草素和鹰嘴豆牙素A.为从中药提取物等复杂体系筛选并鉴定DNA结合剂建立了快速的超滤质谱平台.

Centrifugal ultrafiltration and LC-MS methods were combined to screen DNA-binding agents from traditional Chinese medicine red clover isoflavone extracts.The results show that 10 red clover isoflavones have different binding degrees with DNA.The sample was analyzed by LC-MSn for identifying structures.Among these flavones studied,the strongest five potential DNA-binding agents are irilone-4′-O-β-D-glucoside,glycitin,pratensein,irilone and biochanin A,respectively.A rapid centrifugal ultrafiltration-LC-MS platform for screening and identification of DNA binders from the herbal extract and other complex system was established.