玉屏风散传统饮片汤剂与配方颗粒汤剂对免疫抑制小鼠IL-2、IFN-γ影响的实验研究

Experimental Study of Jade Screen Powder's Prescription Dispensing Granule and Its Decoction of Traditional Cut Crude Drug on Increasing IL-2 and IFN-γ

目的:观察玉屏风散传统饮片汤剂与配方颗粒汤剂对免疫抑制小鼠IL-2、IFN-γ含量的影响,探讨玉屏风散颗粒剂替代传统饮片的可能性。方法:将小鼠随机分为正常对照组、环磷酰胺组(模型组)、玉屏风散饮片低、高剂量组(5,10 g.kg-1)ig、玉屏风散配方颗粒低、高剂量组(5,10 g.kg-1)ig,每日1次,连续10 d。眼球取血,采用ELISA法检测各组小鼠血清中IL-2、IFN-γ的含量。结果:与正常对照组比,环磷酰胺组IL-2、IFN-γ含量明显降低(P<0.01);与环磷酰胺组比,玉屏风散饮片高剂量组和配方颗粒高剂量组IL-2、IFN-γ参数均显著升高(P<0.01),且两组间差异无显著性意义。结论:玉屏风散配方颗粒汤剂升高免疫抑制小鼠IL-2、IFN-γ的参数与传统饮片汤剂相当,玉屏风散配方颗粒汤剂可以替代其传统饮片。

Objective： To observe the effect of Jade Screen Powder in decoction of traditional cut crude drug and in decoction of prescription dispensing granule on IL-2 and IFN-γ, and investigate the substitute possibility of Jade Screen Powder prescription dispensing granule to replace its traditional cut crude drug. Methods： The mice were randomly divided into normal control group, CTX group （ model group）, low and high dose group of Jade Screen Powder＇s cut crude drug （5,10 g ·kg^-1） , low and high dose group of Jade Screen Powder＇s prescription dispensing granule （5, 10 g · kg^-1）. Respectively the mice in each group were given to the medicine once a for 10 days. Then the blood was taken from eyeball, and IL-2 and IFN- γcontent were detected by ELISA. Re- suits： Compared with in normal control group, IL-2 and IFN- γ in high dose group of Jade Screen Powder＇s cut crude drug and in high dose group of Jade Screen Powder＇s prescription dispensing granule were significantly in- creased （P 〈 0.01 ）. And there was not significant difference among the groups. Conclusion： Jade Screen Powder＇s prescription dispensing granule is equivalent to its decoction of traditional cut crude drug on increasing IL-2 and IFN- γ. Jade Screen Powder＇s prescription dispensing granule can replace its decoction of traditional cut crude drug.