干燥方法对龙胆炮制品中龙胆苦苷含量的影响

Influence of Drying Methods on the Content of Gentiopicroside in Gentiana scabra Processed Products

目的:研究干燥方法对龙胆炮制品中龙胆苦苷含量的影响规律。方法:以龙胆苦苷含量为指标,以原药材含量为对照,采用高效液相色谱法测定并比较不同贮藏期阴干、自然晒干、烘干3种干燥方法对龙胆苦苷含量的影响。色谱柱为DikamaC18(250mm×4.6mm,5μm),流动相为甲醇-水(3:7,V/V),柱温为25℃,检测波长为275nm,流速为1.0ml/min,进样量为5μl。结果:自然贮藏过程中,龙胆炮制品中龙胆苦苷含量在贮藏3个月后骤降并随即保持稳定;不同干燥方法所得龙胆苦苷含量:阴干>烘干>自然晒干。结论:虽然干燥方法以阴干最佳,但基于生产实际,建议采用稳定可控的烘干方法。

OBJECTIVE： To study influence of drying methods on the content of gentiopicroside in Gentiana scabra processed products. METHODS ： Using the content of gentiopicroside as index, crude drugs as control, the influence of different drying meth- ods, such as shade drying, natural drying and drying by heat, on the content of gentiopicroside were determined by HPLC. The de- termination was performed on Dikama C18（250 mm×4.6 mm, 5 μm） column with mobile phase consisted of methanol-water （3：7, V/ V） at the flow rate of 1.0 ml/min. The column temperature was set at 25 ℃, and the detection wavelength was set at 275 nm. The injection volume was 5 μl. RESULTS： In the natural storage process, the content of gentiopicroside in G. scabra processed prod- ucts dropped abruptly and kept stable immediately after 3 months of storage. The content of gentiopicroside by different drying methods： shade drying〉drying by heat〉na〉ral drying. CONCLUSION： Shade drying is the best drying method. Based on actual production, it is suggested to use stable and controllable drving by heat.