道地药材黔党参的HPLC指纹图谱研究

Study on HPLC Fingerprints of Genuine Regional Drug Codonopsis tubulosa in Guizhou

目的:建立道地药材黔党参的指纹图谱研究方法,为其质量控制提供理论依据。方法:采用高效液相色谱法。色谱柱为依利特-C18(250mm×4.6mm,5μm),流动相为乙腈-0.2%冰醋酸水溶液(梯度洗脱),流速为0.8ml/min,柱温为30℃,检测波长为270nm。结果:所测10批黔党参样品有13个共有峰,共有峰峰面积的RSD为49.67%～100.93%,峰面积比值的RSD为40.86%～127.97%;共有峰相对保留时间为0.441～1.675,相对保留时间的RSD为0.11%～1.50%;该方法具有良好的稳定性和重复性。结论:所建黔党参HPLC指纹图谱分析方法可较全面地反映该药材中化学成分的信息,可为该药材的质量控制提供理论依据。

OBJECTIVE： To establish the method for fingerprint study of genuine regional drug Codonopsis tubulosa in Gui- zhou. METHODS： HPLC method was adopted. The separation was performed on Hypersil C18（250 mm×4.6 mm, 5 μm）column with mobile phase consisted of acetonitrile-0.2% glacial acetic acid aqueous solution （gradient elution） at the flow rate of 0.8 ml/ min. The column temperature was 30 ℃ and UV detection wavelength was set at 270 nm. RESULTS： There were 13 common peaks of 10 batches of C. tubulosa samples. The RSD of common peak area was from 49.67% to 100.93%. The RSD of relative peak area was from 40.86% to 127.97%. The relative retention time of common peak was 0.441-1.675. The RSD of relative reten- tion time was from 0.11% to 1.50%. The method is of good stability and repeatability. CONCLUSION： Established HPLC chro- matographic fingerprints of C. tubulosa could express its chemical information, and provide a basis for quality control of C tubulo-sa.