依达拉奉对帕金森病小鼠模型中脑细胞自噬的影响

Effect of Edaravone on autophagy in midbrain of Parkinson′s mice model

目的探讨依达拉奉对帕金森病(PD)小鼠脑细胞自噬的影响。方法随机将30只健康雄性C57BL/6小鼠,分为正常对照组、模型组、治疗组各10只。后两组给予腹腔注射百草枯10 mg/kg、代森锰30 mg/kg,每周二、四各1次,连续6周。建模成功后治疗组给予依达拉奉3 mg/kg腹腔注射2周,正常对照组给予等量生理盐水。观察3组小鼠行为学的改变、并用Western blot法检测中脑自噬特异性标记物LC3Ⅱ、beclin 1的表达。结果与治疗组比较,模型组悬挂时间明显缩短,中脑LC3Ⅱ、beclin 1的表达显著增高。结论依达拉奉治疗可显著抑制PD小鼠模型脑细胞自噬。

Objective To investigate the effect of Edaravone on autophagy in midbrain of Parkinson′s disease（PD） mice.Methods Male C57BL/6 mice were randomly divided into the model group,the Edaravone-treated group and the normal control group.The model group and the Edaravone-treated group were intraperitoneally injected with paraquat（10 mg/kg） and maneb（30 mg/kg） twice weekly for 6 consecutive weeks.Subsequent Edaravone-treated group were intraperitoneally injected with edaravone（3 mg/kg） once a day for 2 consecutive weeks.At the same time the normal control group were intraperitoneally injected with the same dose saline.The behavior changes and the motor coordination were observed,and the expressed levels of LC3Ⅱ and beclin1 in the midbrain were detected by Western blot.Results Administration of Edaravone significantly attenuated the impairment of the behavior performance,and decreased the expressions of LC3Ⅱ and beclin1 in the midbrain.Conclusion The present findings suggest that Edaravone has a beneficial influence on parkinsonism by down regulating autophagy in the midbrain.