北刘寄奴中苯乙醇苷类成分的分离与鉴定

Isolation and identification of phenylethanoid glycoside from whole plant of Siphonostegia chinensis Benth.

目的研究中药北刘寄奴(Siphonostegia chinensis Benth.)的化学成分。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱等手段进行分离纯化,并通过理化性质与光谱分析鉴定化合物的结构。结果从北刘寄奴体积分数为95%的乙醇提取物中分离了6个苯乙醇苷类单体成分,分别鉴定为去咖啡酰基类叶升麻苷(decaffeoylverbascoside,1)、acteoside(2)、isoacte-oside(3)、crenatoside(4)、β-oxoacteoside(5)和syringalide A 3'-α-L-rhamnopyranoside(6)。结论其中化合物1、3～6为首次从阴行草属中分离得到。

Objective To study the chemical constituents from whole plant of Siphonostegia chinensis Benth. Methods The compounds were isolated by chromatographic methods such as silica gel, ODS, Sephadex LH- 20 and HPLC, and identified by the chemical and physical methods, especially spectral analysis. Results Six phenylethanoid glycosides were isolated. The structures of them were identified as decaffeoylverbascoside （ 1 ）, acteoside （ 2 ）, isoacteoside （ 3 ）, crenatoside （ 4 ）, fl-oxoacteoside （ 5 ）, and syringalide A 3 ＇-t^-L-rham- nopyranoside（6）. Conclusions Among them,1 and 3-6 are obtained from Siphonostegia genus for the first time.