两面针中化学成分的分离鉴定及活性测定

Isolation,identification and activity determination on the anti-inflammatory components of roots of Zanthoxylum nitidum

目的研究两面针根的化学成分,寻找具有抗炎活性的先导化合物。方法采用硅胶、反相ODS、Sephadex LH-20等柱色谱以及高效液相色谱等手段分离纯化,并通过理化性质和波谱数据鉴定化合物的结构;利用酶标仪测定吸光度,计算样品的一氧化氮(NO)释放抑制率;MTT法测定细胞存活率。结果从该植物中分离得到13个化合物,分别被鉴定为茵芋碱(skimmianine,1)、白鲜碱(dictamnine,2)、zanthobungeanine(3)、zanthodioline(4)、N-methylflindersine(5)、鹅掌楸碱(liriode-nine,6)、L-芝麻素(L-sesamin,7)、β-香树脂醇(β-amyrin,8)、豆甾-9(11)-烯-3-醇(stigmast-9,9)、十三烷胺(tridecane amine,10)、十四烷胺(tetradecane amine,11)、十七烷胺(heptadecanoic amine,12)、十九烷胺(nonadecane amine,13)。结论化合物3、4、8、10～13均为首次从两面针植物中分离得到;体外活性测试结果表明,化合物3具有温和的NO释放抑制活性,其IC50为37.26 mg.L-1。

Objective To study the chemical constituents of roots of Zanthoxylum nitidum （Roxb.）DC. and find compounds with anti-inflammatory activity. Methods Silica gel column chromatography, RP-ODS, Sephadex LH-20 and high performance liquid chromatography were applied for the isolation and purification of the constituents. The structures were elucidated by their physicochemical properties and spectral meth- ods. , The inhibitory activity on NO production and cell survival was evaluated by MTT assay through meas- uring the absorbance using microplate reader. Results Thirteen compounds were isolated, including skimmia- nine （ 1 ）, dictamnine （2）, zanthobungeanine （ 3 ）, zanthodioline （4）, N-methylflindersine （ 5 ）, liriodenine （ 6 ）, L-sesamin （ 7 ）, fl-amyrin （ 8 ）, stigmast-9 （ 11 ） -en-3-ol （ 9 ）, tridecane amine （ 10 ）, tetradecane amine （ 11 ）, heptadecanoic amine （12） and nonadecane amine （13）. Conclusions Compounds 3,4,8,10,11,12 and 13 are all isolated from Zanthoxylum nitidum for the first time. Compound 3 shows moderate NO production inhibi- tory activity with an IC50 value of 37.26 mg.L -1