唑菌酯原药在0.5%羧甲基纤维素钠溶液中的高效液相色谱分析

HPLC Analysis Research of Pyraoxystrobin TC in 0.5% Sodium Carboxymethyl Cellulos

[目的]建立唑菌酯原药在0.5%羧甲基纤维素钠溶液(0.5%CMC)中的高效液相色谱(HPLC)分析方法,测定唑菌酯原药在0.5%CMC中的均一性。[方法]以乙腈-水(体积比70∶30)为流动相,流速为1.0 mL/min,使用Waters Symmetry C18色谱柱,紫外检测,波长254 nm,柱温35℃。[结果]低中高质量浓度样品平均回收率分别为98.0%～99.5%、97.7%～101%和94.9%～98.5%。批内精密度分别为0.1～1.6、0.4～4.1和1.5～2.0。批间精密度分别为0.8、1.7、1.9。线性相关系数大于0.999。配制的低高给药药剂的准确度分别为94.9%～96.4%和101%～103%。[结论]该方法具有简单、可靠、分离效果好、精密度和准确度高、线性关系好的特点,且给药药剂配制准确。

[Aims] A method was developed for the quantitative determination of pyraoxystrobin TC in 0.5% sodium carboxymethyl cellulos （0.5% CMC） under HPLC condition, and the homogeneity of pyraoxystrobin TC in 0.5% CMC was determined. [Methods] The mobile phase was a mixture of acetonitrile and water （70 ： 30, by re1）, and the flow rate was 1.0 mL/min. A Waters Symmetry C18 column and a UV wavelength detector （at 254 rim） were used, and the column temperature was 35 ℃. [Results] For the detection method of pyraoxystrobin in 0.5% CMC, the recoveries for low, middle and high concentration samples were 98.0-99.5%, 97.7-101% and 94.9-98.5%, intra-run precisions were 0.1-1.6, 0.4-4.1 and 1.5-2.0, inter-run precisions were 0.8, 1.7 and 1.9 respectively, and liner correlation coefficients were more than 0.999. The accuracies of low and high concentration formulations were 94.9-96.4% and 101-103%. [Conclusions] The method in this study displayed the advantages of simple, reliable, good separation, high precision and accuracy, and good liner correlations, which made the preparation of the formulations more accurate.