人脐带间充质干细胞移植对肝硬化大鼠TGF-β_1和HGF表达的影响

Effect of human umbilical cord mesenchymal stem cells transplantation on serum TGF-β_1 and HGF expression in liver cirrhosis rats

目的探讨人脐带间充质干细胞(HUC-MSCs)治疗肝硬化大鼠转化生长因子β1(TGF-β1)和肝细胞生长因子(HGF)的表达及机制。方法 40%CCl4大豆油腹部皮下注射制备肝硬化大鼠模型,将肝硬化大鼠随机分成模型组、生理盐水组(NS组)、移植组,每组8只,另设8只作为对照组。移植前、移植后3周分别用Western blot和ELISA法检测大鼠肝组织和血清TGF-β1和HGF水平。同时于光镜下观察肝纤维化程度,计算肝纤维化组织学半定量计分。结果模型组较对照组大鼠肝组织和血清TGF-β1明显升高,HGF明显降低(P<0.05);移植组较模型组TGF-β1明显降低,HGF明显升高(P<0.05)。光镜下,模型组肝组织破坏,假小叶形成;移植组,肝纤维化明显改善。模型组大鼠肝纤维化组织学半定量计分较对照组明显增高(P<0.05);移植组较模型组计分明显降低(P<0.05)。结论 HUC-MSCs移植后可通过下调TGF-β1及上调HGF的表达,改善受损的肝组织。

Objective To investigate transforming growth factor-β1（TGF-β1）and hepatocyte growth factor（HGF） expression and mechanism in liver cirrhosis rats after human umbilical cord mesenchymal stem cells（HUC-MSCs） transplantation.Methods The liver cirrhosis rates were given 40%CCl4 subcutaneous injection in abdomen to make the liver cirrhosis rats model,and then liver cirrhosis rats were randomly divided into model group,NS group and transplantation group,each group with 8 rats,and 8 rats were as control one.Three weeks before and after transplantation,TGF-β1 and HGF levels in the serum and liver tissue were tested by Western blot and ELISA.We tested liver fibrosis degree by microscopy and calculated scores of liver fibrosis.Results TGF-β1 levels in serum and liver tissue were significantly higher in model group than those in control one,however,HGF level was significantly lower（P〈0.05）.TGF-β1 level in transplantation group was significantly lower than that in model group（P〈0.05）.But HGF levels were significantly increased（P〈0.05）.In model group,liver tissues were destruction,false flocculus was formed.Liver fibrosis significantly decreased.Liver fibrosis in model group was significantly higher than that in control group（P〈0.05）,in transplantation group,which was significantly lower than that of model group（P〈0.05）.Conclusion The HUC-MSCs transplantation can improve impaired liver tissue by downregulating TGF-β1 and upregulating HGF.