广西桂北地区慢性HBV感染不同免疫状态与外周血T、B细胞亚群和NK细胞的相关性研究

Study on relationship between different immune states and the levels of peripheral blood T cell subsets, B cell subsets and NK cells in patients with chronic hepatitis B virus infection in North Guangxi

目的了解广西桂北地区慢性HBV感染者不同免疫状态外周血T、B细胞亚群和NK细胞的数值、相互关系及区别。方法分析和比较免疫耐受组、免疫清除组、免疫不全组及对照组共80例慢性HBV感染者,每组20例。用流式细胞仪检测其外周血淋巴细胞亚群和NK细胞,分析其区别与联系。结果百分比和绝对数结果中,免疫清除组的NK细胞、CD4+T细胞及CD4+/CD8+比值较对照组减少(P<0.05),B细胞、CD8+T细胞较对照组增加(P<0.05)。百分比数值中,免疫耐受组和免疫不全组的CD4+T细胞及CD4+/CD8+比值较对照组减少(P<0.05),CD8+T细胞较对照组增加(P<0.05)。绝对值数值中,免疫耐受组和免疫不全组的CD4+T细胞较对照组减少(P<0.05)。不同组间相比较差异有统计学意义(P<0.05)。结论慢性HBV感染者体内存在淋巴细胞亚群失衡和细胞免疫功能紊乱。外周血淋巴细胞和NK细胞的检测对于了解患者免疫状态、评估病情、指导用药和疫苗开发有一定的临床应用价值。

Objective To investigate the relationship between different immune states and the levels of peripheral blood T cell subsets,B cell subsets and NK cells in patients with chronic hepatitis B virus infection in North Guangxi. Methods 80 patients..20 immune tolerance cases, 20 immune clearance cases, 20 immunodeficiency （inactive） cases and 20 normal control cases, were selected. The levels of peripheral blood lymphocyte subsets and NK cells were detected by the flow cytometry. Results In the im- mune clearance group,the numbers of NK cells and CD4＋T lymphocytes, and CD4＋/CD8＋ ratio were lower than those in normal control group（P〈0.05）. And the numbers of B lymphocytes and CD8＋ T lymphocytes were higher than those in normal control group （P%0.05）. In the immune tolerance and immunodeficiency （inactive） group,the number of CD4＋ T lymphocytes and CD4＋/ CD8＋ ratio were lower than those in normal control group （P〈0.05）. And the number of CD8＋T lymphocytes was higher than that in normal control group （P〈0.05）. In the immune tolerance and immunodeficiency （inactive） groups, the number of CD4＋T lymphocytes was lower than that in normal control group （P〈0.05）. There were statistically significant difference between differ- ent groups（P〈0.05）. Conclusion There are lymphocyte subsets imbalance and immune dysfunction in patients with chronic HBV infection. The detection of peripheral blood lymphocyte subsets and NK cells have clinical valuable for understanding the immune status of patients,assessing the patients＇ condition,guiding treatment and developing vaccine.