摘要
目的了解浙江省余姚市感染性腹泻患者中志贺菌感染状况。方法采集感染性腹泻患者的粪便或肛拭标本,进行增菌、分离、生化鉴定、血清分型、药敏试验和PCR毒力基因检测。结果 2007-2011年共检验标本1360份,检出志贺菌36株,检出率为2.65%,其中福氏2a志贺菌4株,占11.11%;福氏2b志贺菌3株,占8.33%;福氏1d志贺菌11株,占30.56%;福氏Xv志贺菌12株,占33.33%;宋内志贺菌6株,占16.67%;检出的志贺菌对多种抗生素耐药。结论 2007-2011年引起余姚市细菌性痢疾主要病原菌是福氏志贺菌,其次为宋内志贺菌;福氏志贺菌有多种血清型,其中Xv血清型分离率最高;药敏试验以氨基糖苷类、喹诺酮类及部分头孢类抗生素敏感性较好;毒力基因呈现多样性。
Objective To understand the infection status among the diarrhea patients in Shigella in Yuyao, Zhejiang province. Methods Stool specimens and anal swabs were collected from infectious diarrhea patients to conduct bacterium enrichment, isolation, biochemical identification, serotyping, drug susceptibility test and virulence gene PCR detection. Results Of 1360 specimens tested in 2007 - 2011, thirty-six were Shigella positive（2. 65% ）. Among of them, four were S. flexneri 2a（ 11.11% ） , three were S. flexneri 2b（8.33% ） , eleven were S. flexneri ld（30. 56% ）, twelve were S. flexneri Xv（33.33% ） and six were Shigella sonnei（ 16. 67% ）. All the Shigella strains were multi-drug resistant. Conclusion From 2007 to 2011, S. flexneri was the major pathogen causing Shigellisis in Yuyao, and S. flexneri Xv is the predominant serotype. Antibiotic susceptibility results indicated that these strains were sensitive to aminoglycosides, quinolones and some cephalosporin. The virulence genes varied.
出处
《疾病监测》
CAS
2013年第1期25-27,共3页
Disease Surveillance
关键词
志贺菌
监测
耐药性
毒力基因
Shigella
surveillance
drug-resistance
virulence gene
