高压氧对弥漫性轴索损伤大鼠脑干水肿及AQP-4表达的影响

Effects of hyperbaric oxygen on the hydrocephalus and expression of Aquaporin-4 in brain stem of rats after diffuse axonal injury

目的:了解高压氧治疗对弥漫性轴索损伤(DAI)大鼠脑干水肿程度及水通道蛋白4(AQP-4)表达的影响,探讨高压氧的脑保护作用的可能分子学机制。方法:采用大鼠头颅瞬间旋转装置制作大鼠DAI模型。经高压氧治疗后采用干湿重法及免疫组化法比较治疗组与损伤组脑干含水量及AQP-4的表达。结果:经高压氧治疗的损伤后6h,1d,3d和5d组大鼠脑干含水量及AQP-4表达与相应时间点DAI组比较均有明显降低(P<0.05)。结论:高压氧治疗可能通过降低脑干AQP-4的表达,从而减轻脑干水肿程度。

Objective：To detect the water content and expression of Aquaporin-4,confirm the relationship between hydrocephalus and AQP-4. And investigate the therapeutic effects of Hyperbaric Oxygen on the Hydroceph- alus and Expression of Aquaporin-4 in Brain Stem of Rats after Diffuse Axonal Injury （DAD. Methods： Experimental studies on the brain diffuse axonal injury （DAD due to instant lateral head rotation. To compare the expressions of AQP-4 and the water content of brain stem between the DAI group and the HBO group by dry-wet weight method and immunohistochemistry after HBO treated. Results：Compared with the DAI group, the expressions of AQP-4 and the water content of brain stem were significantly lower than those in the HBO group（P〈0.05） in 6h, 1d, 3d, 5d af- ter DAI. Conclusion： Hyperbaric oxygen treatment can reduce the water content of brain stem significantly. Its mech- anism is due to the down regulation of AQP-4 possibly.