P物质在慢性中耳炎肉芽及纤维组织中的表达及意义

Expression and the meanings of substance P in the periodontal tissues and the meanings of substance P in the chronic otitis media granulation tissue

目的:探讨P物质(SP)在中耳肉芽组织形成及转归中的作用,揭示神经源性炎症对中耳炎性病变的影响。方法:收集中耳炎患者中耳腔肉芽组织及纤维组织,免疫组化染色,观察SP的表达情况。结果:SP在中耳肉芽及纤维组织中呈阳性表达;SP表达强弱与中耳病变的轻重存在线性相关关系(χ2=25.9919,ν=9,P<0.05)即在中耳病变较重、肉芽范围广者,SP表达高于中耳病变较轻、肉芽范围小者;肉芽组织组SP表达高于纤维组织组("=3.4782,P<0.05)。结论:由SP介导的神经源性炎症反应在肉芽组织形成和发展中起到一定作用;神经原性炎症的强弱影响着中耳腔肉芽组织的增生程度;中耳炎后期,由SP启动的神经源性炎症反应在中耳腔已经减弱。

Objective：Identify the contribution of neuropeptide P to the forming of middle ear granulation tissue, reveals the influence of neurogenic inflammation to otitis media and provides some laboratory proof to under- stand the process and evolution of otitis media. Methods：Collecting granulation tissue and fibrous tissue in middle ear cavity of otitis media patients. Immunity class dyeing and observed the express of neuropeptide P substance. Results： Dyeing of SP situates in granulation tissue and fibrous tissue; Linear relationship between SP and otitis media（χ2= 25. 9919,v=9, P〈0.05）,The expression of SP in the serious affection of middle ear and broader granulation are more than in the slight and small;Dyeing in the group of granulation tissues is heavy than that of fibrous tissues （μ = 3. 4782, P〈0.05）. Conclusion： The neurogenic inflammation mediated by the SP can affect the formation and de- velopment of the granulation tissue; The degree of the neurogenic inflammation can affect the amount of the granula- tion tissue in the middle ear cavity; The neurogenic inflammation mediated by the SP has become weak in the middle ear space during the later period of tympanitis.