紫杉醇脂质体制备及体外抑制两种细胞的作用

Study on the Preparation and Inhibitory Effect on SKOV-3 and HUVEC Cells in vitro of Paclitaxel Liposomes

目的:制备紫杉醇脂质体并考察体外对人卵巢癌上皮细胞(SKOV-3)和人脐静脉内皮细胞(HUVEC)两种细胞的抑制作用。方法:采用薄膜分散法制备紫杉醇脂质体,透射电镜观察脂质体的形貌,激光粒度仪测量粒径和Zeta电位,MTT法检测脂质体体外对SKOV-3和HUVEC的抑制作用。结果:制备的脂质体呈圆形或类圆形,平均粒径为54.6 nm,Zeta电位为-40.9 mv,体外释放符合Higuchi方程。体外细胞结果实验显示,紫杉醇能同时抑制肿瘤细胞和血管内皮细胞的生长。结论:紫杉醇脂质体较紫杉醇注射液(TAXOL)能更有效的抑制SKOV-3和HUVEC的生长。

Objective：To prepare paclitaxel liposomes and study the inhibitory effect on SKOV-3 and HUVEC in vitrd.Method： Paclitaxel liposomes were prepared by film dispersion method.TEM was used to observe the morphology of the liposomes,and the particle size and potential were measured by a laser particle size analyzer.MTT experiment was adopted to evaluate the inhibitory effect of paclitaxel liposomes on SKOV-3 and HUVEC cells.Result;Paclitaxel liposomes showed round or oval morphology with the average particle size of 54.6 nm and Zeta potential of -40.9 mv.In vitro drug release was conformed to a Higuchi equation.The results of in vitro experiments suggested that paclitaxel liposomes had the ability to inhibit the growth of tumor cells and vascular endothelial cells. Conclusion：Paclitaxel liposomes possess higher inhibitory effect on SKOV-3 and HUVEC than paclitaxel injections（TAXOL）.