摘要
目的:对不同品种的甘草总黄酮进行对比分析。方法:以甘草苷作为对照品采用三波长测定法对不同品种的甘草总黄酮进行定量分析。结果:选取的三个检测波长分别为282.0,346.2,358.0 nm,线性方程为△A′=0.357 1C+0.016,r=0.999 7,说明总黄酮的浓度在6.012～60.125μg·ml^(-1)范围内,△A′与浓度之间呈较好的线性关系,可按标准曲线进行定量分析,本方法平均回收率为99.91%。结论:三波长测定法操作简便、快速、准确,具有良好的分析应用前景;不同品种甘草中总黄酮含量有较大差异,其中野生胀果甘草中总黄酮含量最高,野生光果甘草和野生黄甘草含量次之,野生和栽培的植物甘草总黄酮含量最低。
Objective：To compare the content of total flavonoids in five varieties of licorice.Method：A three-wavelength spectrophotometry was applied in the determination of total flavonoids in licorice.Result：The detection wavelengths were 282.0,346.2 and 358.0 nm.The linear equation was△A＇-0.357C ＋ 0.016（r = 0.999 7） within the concentration of total flavonoids of 6.012- 60.125μg ？ ml^（-1）.△A＇ and concentration C had good linear relationship which could be used in the quantitative analysis.The average recovery was 99.91%.Conclusion：The method is simple,rapid and accurate with good application prospect.There is significant difference in total flavonoids in various varieties of licorice,and the highest content of total flavonoids is found in wild glycyrrhiza inflata.
出处
《中国药师》
CAS
2013年第1期49-51,共3页
China Pharmacist
关键词
甘草
总黄酮
三波长测定法
Licorice
Total flavonoids
Three-wavelength spectrophotometry
