摘要
目的:建立HPLC测定二维亚铁颗粒(Ⅱ)中维生素C和维生素B_6含量的方法。方法:采用Insteril ODS-3色谱柱(250 mm×4.6 mm,5μm),流动相:0.03 mol·L^(-1)磷酸氢二铵溶液(加入0.01%庚烷磺酸钠,用磷酸调节pH至3.0)-甲醇,梯度洗脱,检测波长为280 nm,流速:0.7～1.0 ml·min^(-1),柱温30℃。结果:维生素C与维生素B6分别在37～594μg·ml^(-1)(r=0.999 9)与3～51μg·ml^(-1)(r=1.000 0)范围内线性关系良好,平均回收率分别为99.6%与99.2%,RSD分别为0.48%与0.54%(n=9)。结论:本法简便、结果准确、可靠,可用于二维亚铁颗粒(Ⅱ)中维生素C、维生素B_6的含量测定。
Objective：To establish an HPLC method for the determination of vitamin C and vitamin B_6 in ferrous fumarate granules. Method：The procedure was performed on an Insteril ODS-3 column（250 mm×4.6 mm,5μm）at 30t with the detection wavelength at 280 nm.The mobile phase was composed of 0.03 mol ？ L~＇ ammonium phosphate solution（0.01%sodium heptanesulfonate was added and the pH was adjusted to 3.0 by phosphoric acid） and methanol with gradient elution,and the flow rate was 0.7-1.0 ml ？ min^（-1）.Result：Vitamin C and vitamin B_6 were in good linearity within the range of 37-594μg ？ ml^（-1）（r =0.999 9） and 3-51μg·ml^（-1）,（r = 1.000 0）,respectively.The mean recovery was 99.6%and 99.2%with RSD of 0.48%and 0.54%（n= 9） accordingly. Conclusion：The method is simple,accurate,reliable and appropriate in the determination of vitamin C and vitamin B_6 in ferrous fumarate granules.
出处
《中国药师》
CAS
2013年第1期84-87,共4页
China Pharmacist
