黄精多糖的提取及其对小鼠免疫活性的影响

Extracting of Polygonatum Polysaccharides and Effecting on the Immunological Activity in Immunosuppressed Mice

目的:提取黄精多糖,观察黄精多糖对免疫抑制小鼠免疫功能的影响,为其开发应用提供试验依据。方法:采用水溶剂煎煮提取、乙醇沉淀及Sevage法去蛋白制备黄精多糖。腹腔注射环磷酰胺,建立小鼠免疫抑制模型,测定低、高(200、600mg/kgbw)剂量黄精多糖(灌胃给药)对模型小鼠的免疫器官质量、血清溶血素和腹腔巨噬细胞吞噬功能的影响。结果:黄精多糖的提取率为(9.20±0.15)%,纯度为(84.65±1.31)%。与模型对照组比较,黄精多糖可提高免疫抑制小鼠的脾脏、胸腺指数,显著促进溶血素的形成(P<0.05),显著提高腹腔巨噬细胞吞噬鸡红细胞的吞噬率和吞噬指数(P<0.05)。结论:黄精的多糖含量高。黄精多糖可明显改善环磷酰胺致免疫抑制小鼠的免疫功能,提示黄精多糖具有开发价值。

Objective： To extract the polysaccharides from polygonatum and to investigate the effects of the polygonature polysaccharide （PP） on immunological function in immunosuppressed mice, and provide the experimental basis for develop and application of PP. Methods： The PP was extracted from dried roots of polygonatum by hot water extraction, ethanol precipitation and deproteinization with Sevage method. The mice were established as immunosuppressed model by peritoneal injecting cyclophosphamide. The mice were administered once daily by intragastric administration with PP solution dose of 200 and 600 mg/kg respectively for 7 days. The effects of PP on model mice were measured following these indexes, the weights of immune organs, the serum hemolysin, and phagocytic activity of peritoneal macrophages. Results： The extraction rate of PP was（9.20＋0.15）% and content（84.65＋l.31）% in the condition of the ratio of solid to liquid 1： 15, extraction temperature 90 ~C and time 2 h for two times, While compared with immunosuppressive model mice groups, the PP groups can increase the thymus and spleen indexs, phagocyte-percentage and phagocytic-index of peritoneal macrophages significantly（P〈0.05）, and can promote the formation of hemolysin significantly（P〈0.05）. Conclusions： The PP can obviously improve immunological function of the immunosuppressive model mice induced with cyclophosphamide. The polygonatum contain high content of polysaccharide that is worthy of development.