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慢病毒介导Bcl-2基因保护化疗大鼠卵巢损伤的作用及机制 被引量：3

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摘要 目的探讨携带Bcl-2基因的慢病毒局部注射保护环磷酰胺(CTX)损伤大鼠卵巢的作用及机制。方法将Wistar大鼠分为7组,每组16只,分别为空白对照组;CTX组(仅腹腔注射CTX);Bcl-2组;增强型绿色荧光蛋白(EGFP)组;盐水+CTX组;空载+CTX组;实验组(注射pGC-FU-Bcl-2+CTX);将纯化的慢病毒悬液经开腹注射入双侧卵巢内,部分动物采用CTX经腹腔注射的方式建立卵巢早衰动物模型。采用HE病理切片计数各级卵泡数量,Tunnel法检测卵巢组织中颗粒细胞凋亡情况,Western blot检测Bcl-2蛋白定量。结果卵巢内直接注射慢病毒后,实验组各级卵泡数比空载+CTX组、盐水+CTX组及CTX组均明显增加,抗凋亡蛋白Bcl-2明显增加,卵巢颗粒细胞凋亡率明显下降。结论携带Bcl-2基因的慢病毒可防护CTX对卵巢组织结构的损伤,其发挥作用的途径之一则是上调Bcl-2蛋白,抑制卵巢颗粒细胞凋亡。
作者 王雪峰 何援利
机构地区 南方医科大学珠江医院妇产科
出处 《广东医学》 CAS CSCD 北大核心 2013年第2期187-191,共5页 Guangdong Medical Journal
基金 国家自然科学基金资助项目(编号:81041101) 广东省自然科学基金资助项目(编号:10451051501004704)
关键词 BCL-2基因 慢病毒 化疗药物 卵巢损伤
分类号 R-332 [医药卫生]
  • 相关文献

参考文献16

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二级参考文献34

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共引文献67

同被引文献44

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引证文献3

二级引证文献40

广东医学
2013年 第2期

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