甘蓝型油菜“甘杂1号”种子纯度的SSR鉴定

Hybrid purity identification of "Ganza No.1" using SSR method

【目的】建立有效的鉴定"甘杂1号"种子纯度的SSR分子标记方法,为提高"甘杂1号"大田制种纯度提供分子辅助工具。【方法】以"甘杂1号"杂交种及其母本3131A和父本3116C种子为供试材料,利用SSR分子标记技术鉴定"甘杂1号"种子纯度,从200对引物中筛选出特异性强、稳定性高的SSR引物,并将SSR引物鉴定的"甘杂1号"种子纯度与田间种植鉴定结果进行比较。【结果】在200对SSR引物中,筛选出了2对在杂交种和父母本之间存在显著差异的引物(CB10569和Na12E03),且条带清晰可辨,即杂交种含有父母本的特异互补带型。将这2个引物在120株"甘杂1号"杂交种群体中进行重复验证,发现CB10569和Na12E03 2对引物鉴定出"甘杂1号"的种子纯度分别为82%,79.5%。SSR标记的鉴定结果与田间种植鉴定结果(83%)基本一致。【结论】得到的2对SSR引物CB10569和Na12E03,可用于甘蓝型油菜品种"甘杂1号"种子纯度的鉴定。

[Objective] This study established a rapid and reliable SSR method to identify the purity of hybrid ＂Ganza No. 1＂, which provided an auxiliary tool to improve the field purity of ＂Ganza No. 1＂. [Method] The purity of Brassicanapus cultivar ＂Ganza No. 1＂ was identified by SSR marker,and the re- sults were compared with the field planting appraisal in order to screen out SSR primers with high stabili- ty. [Result] Among the 200 pairs of SSR primers screened,2 pairs of primers （CB10569 and Na12E03） had significant and legible differences between hybrid seeds and their parents. In other words, the bands ampli- fied from the hybrid seeds contained complementary bands of both parents＇ specific bands. 120 individual seeds of ＂Ganza No. 1＂ were tested with the selected primers （CB10569 and Na12E03）,and the seed puri- ties detected were 82% and 79.5% ,respectively,which were close to the result of 83% identified in the field. [Conclusion] SSR primers CB10569 and Na12E03 could be used to identify the seeds purity of ＂Ganza No. 1＂