IL-17对黑色素瘤B16F10细胞增殖和侵袭的影响

Effects of IL-17 on proliferation and invasion of B16 F10 melanoma cells

目的:探讨白细胞介素-17(IL-17)对小鼠黑色素瘤B16F10细胞增殖和侵袭的影响。方法:体外培养小鼠黑色素瘤B16细胞,选择对数生长期的细胞,在培养基中加入一定浓度的IL-17,并设空白对照组,MTT法检测IL-17对B16细胞增殖能力的影响;transwell侵袭实验检测IL-17对B16细胞侵袭能力的影响;明胶酶谱法分析IL-17处理后细胞MMP-2和MMP-9的表达活性。结果:IL-17处理组与对照组相比,B16细胞的增殖能力无变化(P>0.05)。transwell侵袭实验显示,IL-17处理组B16细胞穿膜细胞数明显增多(P<0.01)。明胶酶谱实验显示,IL-17处理后细胞MMP-2和MMP-9的表达活性增强(P<0.01)。结论:IL-17对黑色素瘤B16F10细胞的增殖能力无影响,但可促进细胞侵袭,可能通过增强MMP-2和MMP-9的活性而发挥作用。

Objective： To investigate the influence of interlukin-17 （IL-17） on the proliferation and invasion of mouse B16F10 melanoma cells. Methods： Mouse B 16 melanoma cells were eultured in vitro. Cells in researeh group were treatead with a certain-coneentration IL-17 and those in eontrol group treated without IL-17.The proliferation of B16 cells was detected by MTT. And the invasieness of B16 cells was determined by transwell. Then the expression activities of MMP-2 and MMP-9 of cells were mesurcd Gelatin Zymography. Results： The growth rate of B 16 cells treated with IL-17 was not changed （P〉0.05） eompared with the control group. Matrigel invasion assay revealed that addition of IL-17 to B I6 cells stimulated marked invasion （P〈0.01）. And Gelatin Zymography analyzed that the expression of MMP-2 and MMP-9 of cells was significantly enhanced in the researeh group（P〈0.01）. Conclusion： IL-17 has no effect on proliferation of BI6F10 cells but can promote the invasion whieh may due to the increased MMP-2 and MMP-9 activities.