摘要
目的观察白细胞介素-17（IL-17）在糖尿病大鼠肾组织中的表达，探讨其与肾小管-间质病变的关系。方法90只大鼠随机分为对照组（Con组）、1型糖尿病组（T1DM组）及2型糖尿病组（T2DM组），以50mg／kg链脲佐菌素（STZ）制备1型糖尿病模型，以高脂高糖饮食及25mg／kgSTZ制备2型糖尿病模型。于2周、4周末采用酶联免疫吸附试验（ELISA）检测血、尿中IL-17水平；4周末采用免疫组织化学染色及Westernblot检测肾组织中IL-17及转化生长因子-B1（TGF-131）水平。结果糖尿病大鼠2、4周末m、尿中IL。17升高；IL-17在Con组。肾组织无表达，仅在糖尿病大鼠肾小管-问质中表达，TIDM组0．2357±0．0410，T2DM组0．1809±0．0090（P〈0．05）；TGF-131肾组织表达，T1DM组0．1412±0．0610，T2DM组0．2809±0．0420（P〈0．05）；肾组织IL-17与尿液IL-17水平呈正相关（r=0．715，P〈0．05）。结论IL-17参与糖尿病肾小管-问质病变的发生；IL-17和TGF-／B1在1型和2型糖尿病发展为糖尿病肾病中的作用机制存在差异。
Objective To investigate the association between the expression of interleukin-17 （ IL-17 ） in renal tissues of rats with diabetic nephropathy and tubulointerstitial lesions. Methods Ninety rats were randomly divided into three groups： control group, type 1 diabetic mellitus （T1DM） group, and type 2 diabetic mellitus （T2DM） group. The rats in T1DM group were given 50 mg/kg streptozotiein （STZ） , and those in T2DM group fed on a diet enriched with sucrose, triglyceride and cholesterol, and in- jected with 25 mg/kg STZ. At 2nd and 4th week, serum and urine IL-17 levels were determined by using enzyme-linked immunosorbent assay （ELISA）. The expression of IL-17 and transforming growth factor-131 （ TGF-B1 ） in the kidney was detected by using immunohistoehemisty staining and Western blotting. Results Serum and urine IL-17 levels were obviously increase in diabetic rats. The expression of IL-17 was detectable in renal tubulointerstitium of diabetic rats. The expression of IL-17 in T2DM and T1DM groups was 0. 1809 ±0. 0090 and O. 2357 ±0. 0410 respectively （P 〈0. 05）. The expression of TGF-B1 in T2DM and T1DM groups was 0. 2809 ± 0. 0420 and 0. 1412 ± 0. 0610 respectively （ P 〈 0.05 ）. The expression of IL-17 in renal tubulointerstitium of diabetic rats was positively correlated with urine IL-17 （r = 0. 715 ,P 〈 0. 05 ）. Conclusion The increase of IL-17 expression may play an important role in diabetes mellitus-in- dueed tubulointerstitial lesions. IL-17 and TGF-[31 might exert different effects on pathogenesis and pro- gression of diabetic nephropathy from T1DM and T2DM.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第2期342-344,F0004,共4页
Chinese Journal of Experimental Surgery
基金
湖北省自然科学基金资助项目（2012FFB04306）
