血管紧张素-Ⅱ腹腔注射建立小鼠主动脉夹层动物模型

Establishment of mouse aorta dissection model through intraperitoneal injection of angiotensin II

目的应用血管紧张素-Ⅱ（Ang-Ⅱ）腹腔注射建立小鼠主动脉夹层模型。方法8月龄C57BIM6J小鼠40只，雌雄不拘，随机分为2组。实验组20只，每8h腹腔注射血管紧张素Ⅱ，剂量为每天4．5mg／kg；对照组20只，每8h腹腔注射去甲肾上腺素，剂量为每天12mg／kg。分别于注射30min后应用鼠尾测压器检测并记录小鼠血压（收缩压）。精心饲养14d，中途死亡小鼠直接解剖，取出主动脉。14d后，存活小鼠颈椎脱臼处死，解剖取出主动脉，大体及光镜观察。结果实验组小鼠检测血压为（134．5±3．5）mmHg（1mmHg=0．133kPa），对照组检测血压为（132．9±2．7）mmHg，两组血压差异无统计学意义（P〉0．05）。实验组中有7只小鼠大体标本观察有主动脉夹层发生，另有12只标本光镜下观察发现内膜明显增厚，中膜变薄，可见断裂，符合主动脉夹层组织病理学表现。对照组所有标本大体及光镜下观察均无明显组织病理学改变。结论腹腔注射Ang-Ⅱ能建立小鼠主动脉夹层模型。

Objective To establish the mouse aorta dissection model through intraperitoneal injec- tion of angiotensin ]I ~ Methods Forty 8-month-old C57BL/6J mice without gender limitaiton were ran- domly divided into two groups （n = 20 each）. The animals in experimental group received intraperitoneal injection of angiotensin ]I every 8 h, 4. 5 mg/kg every day, and those in control group received intraperito- neal injection of noradrenaline every 8 h, 12 mg/kg every day. Thirty min after injection, blood pressure was measured using tail cuff blood pressure device （systolic pressure）. All mice were fed carefully for 14 days. If mice died during this period, they were anatomized directly in order to take aorta tissue. Fourteen days later, the rest mice were killed and aorta walls were taken to observe the structural changes pathologi- cally. Results The blood pressure in experimental group was （ 134. 5 ± 3.5） mm Hg （ 1 mm Hg =O. 133 kPa）, and （ 132. 9 ±2. 7） mmHg in control group respectively （P 〉0. 05）. In experimental group, the tunica media of arteriae aorta walls of 12 mice were thinner and some of them were broken. Also, obvi- ous aortic dissection was found in 7 mice of experimental, but no significant pathological changes were found in control group. Conclusion The mouse aorta dissection model can he established effectivly through intraDeritoneal injection of anziotensin 11.