组织芯片检测肝肠钙粘连蛋白在肝癌组织中的表达及临床意义

Differential expression of liver-intestine cadherin in hepatocellular carcinoma and its clinical significance

目的研究肝肠钙粘连蛋白(LI-cadherin)在肝癌组织中的表达以及与肝癌的发生发展和临床病理特征之间的相关性。方法采用免疫组织化学SP法检测70例不同类型肝癌组织和5例正常肝组织标本中LI-cadherin的表达情况,并结合临床资料和相关病理参数进行统计分析。结果 70例肝癌组织中LI-cadherin阳性表达39例,总阳性率为55.7%,5例正常肝组织中未见阳性表达;LI-cadherin的表达与年龄、性别、肿瘤分化程度以及有无远处转移无关(P>0.05),而与淋巴结转移及血管侵犯密切相关(P<0.05)。结论 LI-cadherin的表达与肝癌的发生、转移和浸润有关,LI-cadherin可能成为诊断肝癌发生、转移及临床分期的新生物学标志物,对于提高临床治疗效果具有指导意义。

Objective To investigate the expression of liver - intestine （LI） - cadherin in hepatocellular carcinoma （HCC） by tissue microarray and explore its relationship with pathologic features of HCC patients. Methods Seventy primary HCC resection samples with different indexing and five primary normal tissue samples were assessed by tissue microarray and immunohistochemistry based on the SP method. The detected expression levels of LI - cadherin were compared to the clinic pathologic parameters of the tissue donors. Results LI - cadherin expression was detected in 39 （55.7%） of the 70 primary HCC tissues, and none of the normal tissues. Positivity for LI -cadherin expression was significantly associated with lymph node metastasis and venous invasion （ both P 〈 0.05 ）, but no significant association was observed with age, sex, tumor grade, or metastasis （ all P 〉 0.05 ）. Conclusion LI - cadherin expression may be associated with HCC occurrence, tumor invasion, and metastasis. Future studies should assess the potential of LI - cadherin expression as a diagnostic biomarker or target of molecular therapy for I-ICC.