沙丁胺醇多克隆抗体的制备及间接ELISA方法的建立

Preparation of salbutamol polyclonal antibodies and development of indirect competitive enzyme-linked immunoassay

目的:制备特异性好的沙丁胺醇多克隆抗体,优化并建立间接ELISA快速检测沙丁胺醇(SAL)浓度的方法。方法:采用小剂量长周期的方式免疫新西兰大白兔,优化ELISA检测方法,检测一系列SAL浓度,建立标准曲线,并对方法的精密度和回收率进行测定。结果:所制备的沙丁胺醇多克隆抗体的IC50为12.21 ng/ml,建立了间接ELISA检测SAL浓度的标准曲线,样本的回收率在95%～105%之间,变异系数﹤3%。结论:本研究建立了快速、灵敏的间接ELISA检测SAL浓度的方法。

Objective： To prepare the antibodies against salbutamol （SAL） with high sensitivity and to develop an indirect competitive enzyme-linked immunoassay （ic-ELISA） for fast detection of SAL. Methods： The New Zealand white rabbits were immunized with SAL in a small close and long period mode. The method of ie-ELISA was optimized and adopted for the detection of a series of SAL samples, then the standard curve of SAL was established. The precision and the recoveries of the method were determined. Results： The antibodies with high sensitivity towards SAL were prepared with a IC50 of 12.21 ng/ml. The ie-ELISA method for SAL measurement was established, the recoveries of measurement was between 95% - 105% and the CV was 〈 3%. Conclusions： The antibodies against salbutamol have been prepared and an indirect competitive enzyme-linked immunoassay for fast and specific detection of SAL has been developed.