摘要
目的:制备特异性好的沙丁胺醇多克隆抗体,优化并建立间接ELISA快速检测沙丁胺醇(SAL)浓度的方法。方法:采用小剂量长周期的方式免疫新西兰大白兔,优化ELISA检测方法,检测一系列SAL浓度,建立标准曲线,并对方法的精密度和回收率进行测定。结果:所制备的沙丁胺醇多克隆抗体的IC50为12.21 ng/ml,建立了间接ELISA检测SAL浓度的标准曲线,样本的回收率在95%~105%之间,变异系数﹤3%。结论:本研究建立了快速、灵敏的间接ELISA检测SAL浓度的方法。
Objective: To prepare the antibodies against salbutamol (SAL) with high sensitivity and to develop an indirect competitive enzyme-linked immunoassay (ic-ELISA) for fast detection of SAL. Methods: The New Zealand white rabbits were immunized with SAL in a small close and long period mode. The method of ie-ELISA was optimized and adopted for the detection of a series of SAL samples, then the standard curve of SAL was established. The precision and the recoveries of the method were determined. Results: The antibodies with high sensitivity towards SAL were prepared with a IC50 of 12.21 ng/ml. The ie-ELISA method for SAL measurement was established, the recoveries of measurement was between 95% - 105% and the CV was 〈 3%. Conclusions: The antibodies against salbutamol have been prepared and an indirect competitive enzyme-linked immunoassay for fast and specific detection of SAL has been developed.
出处
《浙江大学学报(医学版)》
CAS
CSCD
北大核心
2013年第1期19-24,共6页
Journal of Zhejiang University(Medical Sciences)
基金
浙江省重大科技专项基金(2010C13006)
浙江省卫生科研基金(2011KYA044)
关键词
沙丁胺醇
酶联免疫吸附测定
佐剂
免疫
抗体
多克隆
分析
Albuterol
Enzyme-linked immunosorbent assay
Adjuvants, immunologic
Antibodies,polyclonal/analysis