小麦^(60)Coγ射线辐照后TaKu70和TaKu80基因应答模式及基因组多态性分析

TaKu70 AND TaKu80 GENES RESPONSE PATTERNS AND GENOMIC POLYMORPHISM ANALYSIS OF WHEAT IRRADIATED BY GAMMA RAYS

本研究以冬小麦品种京411(J411)和邯6172(H6172)为材料,探讨60Coγ射线辐照后辐照敏感性、TaKu70和TaKu80基因应答模式与M1代基因组AFLP多态性频率之间的关系。结果表明,60Coγ射线辐照对小麦的生长具有明显抑制作用,其中J411的辐照敏感性高于H6172。在J411和H6172中TaKu80基因对60Coγ射线辐照做出表达量上调的应答,但TaKu70基因的表达则没有明显变化,其中J411中TaKu70和TaKu80基因的表达水平低于H6172。60Coγ射线辐照导致J411和H6172的M1代基因组多态性频率升高,其中J411中多态性频率上升的水平明显高于H6172。由此可见,TaKu70和TaKu80基因的表达水平及辐照应答模式与小麦品种辐照敏感性和辐照后代基因组多态性具有密切关系,有可能成为改善小麦品种辐照敏感性、提高诱变育种效率研究中极具应用潜力的新途径。

60Co γ-rays irradiated seeds and seedling of two wheat genotypes Jing411（J411） and Han6172（H6172） were used to study the relationship among radiation sensitivity,response patterns of TaKu70 and TaKu80 genes and the genomic AFLP polymorphism in the M1 generation.The results showed that γ-rays irradiation significantly inhibited the seedling growth of wheat,and the radiation sensitivity of J411 was higher than that of H6172.TaKu80 gene expression was up-regulated in response to γ-rays irradiation in J411 and H6172,but no significant changes in the expression level of TaKu70 gene.The expression level of TaKu70 and TaKu80 genes in J411 were lower than in H6172.γ-rays irradiation could increase the genomic polymorphism of M1 in J411and H6172,and the polymorphic frequencies increased in J411 was significantly higher than that in H6172.It was concluded that the expression level and response pattern of TaKu70 and TaKu80 genes were closely related with wheat radiation sensitivity and irradiated genomic polymorphism.Transformation of TaKu70 and TaKu80 genes may be a new approach to improve the radiation sensitivity,and to improve mutation breeding efficiency in wheat.