利用CAPS标记推测花生品种(系)FAD2位点基因型的研究

IDENTIFICATION OF FAD2 GENOTYPE FOR PEANUT CULTIVARS AND STRAINS BY CAPS MARKER

以5个高油酸含量花生品种(系)和2个普通油酸含量花生品种为试材,利用CAPS标记对这些品种(系)的FAD2位点基因型进行分析。结果表明:5个高油酸含量花生品种(系)的油酸含量均在80%以上,普通油酸含量品种花育22号和花育28号油酸含量分别为51.62%和41.38%。基于CAPS标记在7个品种(系)中的扩增产物及酶切带型分析推测,AhFAD2A位点花育28号为野生型,其他6个品种(系)符合448 G>A突变类型;AhFAD2B位点花育28号和花育22号为野生型,开农H03-3、花育32号、P76和F18符合441-442insA突变类型,而06B16在该位点不符合441-442insA突变类型。

In this study,five high-oleate cultivars（strains） and two normal-oleate cultivars were selected as materials.FAD2 genotype of these cultivars and strains were analyzed.The results showed that the oleic acid content of five high-oleate cultivars and strains exceeded 80%.The oleic acid content of normal-oleate cultivars（Huayu 22 and Huayu 28） were 51.62% and 41.38%,respectively.According to the amplicons and digested products,i.e CAPS marker analysis,we deduced that Huayu 28 had wild-type alleles of ahFAD2A,while other six cultivars（strains） had mutant allele for the CAPS of 448GA transition.For another CAPS of the ahFAD2B,abase pair insertion（441-442）,Huayu 28 and Huayu 22 had the wild-type allele,while ahFAD2B of high-oleate cultivars（strains） except 06B16 had the mutant allele.