超声波酶法提取红松树皮中多酚类化合物的研究

Study on ultrasonic-enzymatic extraction of polyphenolic compounds in Korean pine bark

采用超声波酶法对红松树皮中多酚类化合物的提取工艺进行研究,以多酚得率、DPPH·清除率为指标,在单因素实验基础上通过正交实验优化最佳工艺条件。结果表明:超声波酶法的最佳工艺条件是:超声温度60℃,超声时间80min,超声功率250W,酶解温度45℃,酶添加量4.0%,酶解时间70min和pH4.8。此时,多酚提取量达到78.79mg/g,DPPH·清除率为63.68%。采用正交实验法优化红松树皮多酚的超声波酶法提取工艺,具有可行性,且此工艺提取的多酚得率较高及具有良好的抗氧化活性。

The ultrasonic-enzymatic extraction process was used to extract craft of polyphenols in Korean pine bark. The total polyphenol yield,DPPH radical scavening was used to indicate. The optimum conditions was obtained through orthogonal test based on single factor experiment.The results showed that the optimal ultrasonic- enzymatic extraction condition was as follows：ultrasonic temperature 60℃,ultrasonic time 80min,ultrasonic power 250W,enzyme solution temperature 45℃,adding amount of enzyme 4.0% ,enzyme solution time 70min and pH4.8. The polyphenol extraction rate was 78.79mg /g and the DPPH radical scavening ratio was 63.68% .The method of the orthogonal test used to optimize the extraction craft of the ultrasonic-enzymatic extraction of the polyphenols in Korean pine bark was available. Under the optimum condition,the polyphenols in Korean pine bark had higher antioxidant activity.